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Data show that recombinant AGO3 loaded with miR (显示 MLXIP 蛋白)-20a cleaves complementary target RNAs.
Study uncovers a first example of a vertebrate protein factor, Argonaute-3, specifically affecting the guide-to-passenger-strand ratio of the miRNA let-7a. A multi-layered mechanism for the observed impact of Ago3 on the let-7a-3p passenger strand expression and activity is proposed.
The N-terminal domain of Ago3 inhibits cleavage activity. AAs (显示 FGD1 蛋白) 1-64 are largely unstructured & form a large loop surrounding an area containing two helices & another unstructured loop.
The Ago3 PIWI (显示 PIWIL1 蛋白) domain is slicing competent but our Ago3 model suggests multiple interactions between residues in the PIWI (显示 PIWIL1 蛋白) & N domains that may increase protein rigidity or invoke other properties that contribute to Ago3's observed slicing deficiency.
DICER (显示 DICER1 蛋白)- and AGO3-dependent generation of retinoic acid-induced DR2 Alu RNAs regulates human stem cell proliferation.
Ago3 is able to load microRNAs efficiently in the absence of Ago1 (显示 EIF2C1 蛋白) and Ago2 (显示 EIF2C2 蛋白), despite a significant loss of global microRNA expression
reliable predictions of miRNA affinity to Ago2 (显示 EIF2C2 蛋白) AND Ago3 proteins were made.
The specificity of RNA interference depends on the concentration of Ago1 (显示 EIF2C1 蛋白), Ago3, and Ago4 (显示 EIF2C4 蛋白) relative to Ago2 (显示 EIF2C2 蛋白).
EIF@C3 protein (显示 RPS3A 蛋白) is expressed in both Schwann and neuron-type differentiating cells.
Results from the liver show that, siRNA targets 3'UTR and the coding sequence (CDs (显示 ABHD5 蛋白)) of endogenous genes in the presence Ago2 (显示 EIF2C2 蛋白) but in its absence, only 3'UTR-targeted siRNA-mediated knockdown are active with the help of Ago1 (显示 EIF2C1 蛋白) and Ago3.
enhanced flu susceptibility of Ago1 (显示 EIF2C1 蛋白)/3 double-knockout mice arises from an intrinsic impairment in the ability of lung cells to tolerate flu-elicited inflammation.
EIF2C2 (显示 EIF2C2 蛋白)-4 and PIWIL4 (显示 PIWIL4 蛋白) appear increased in advanced tumors with distant metastasis, suggesting they may promote tumor invasion
Data show that Ago4 (显示 EIF2C4 蛋白)- Ago1 (显示 EIF2C1 蛋白)-Ago3 genes are linked together at the p12 (显示 SPRN 蛋白) of the chromosome 6, while Ago2 (显示 EIF2C2 蛋白) is located at the p15 (显示 CDKN2B 蛋白) of the chromosome 4.
Data show that Argonaute family protein AGO2 (显示 EIF2C2 蛋白) and AGO3 were important for abscisic acid (ABA)-mediated resistance.
AGO3 could not complement the signature function of AGO2 (显示 EIF2C2 蛋白), the closest genetic paralog of AGO3, in host antiviral defence. It was also found, surprisingly, that AGO3 predominantly bound 24-nt sRNAs with 5'-terminal adenine. The spectrum of AGO3-associated sRNAs was different from those bound to AGO2 (显示 EIF2C2 蛋白), further indicating their functional divergence.
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene.
, eIF-2C 3
, eIF2C 3
, eukaryotic translation initiation factor 2C, 3
, protein argonaute-3
, argonaute 3b
, argonaute RISC catalytic component 3
, eukaryotic translation initiation factor 2C, 3b
, Piwi/Argonaute family protein meIF2C3
, eukaryotic translation initiation factor 2C 3