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|抗原||Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) 抗体|
|抗原表位||AA 117-250 其他选择|
|标记||This EIF2AK2 antibody is un-conjugated 其他选择|
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|Supplier||Log in to see|
EIF2AK2抗体详情EIF2AK2目标详情 使用细节 贮存及处理 References for anti-EIF2AK2 antibody (ABIN968165) 图像
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|纯化方法||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|免疫原||Human p68 Kinase aa. 117-250|
EIF2AK2目标详情EIF2AK2抗体详情 使用细节 贮存及处理 References for anti-EIF2AK2 antibody (ABIN968165) 图像 回页首
|别名||PKR (EIF2AK2 Antibody 摘要)|
Double stranded RNA (dsRNA) generated by most viruses during the infectious cycle is a potent stimulator of interferons. Interferons bind to cell surface receptors and stimulate the synthesis of several proteins that interfere with viral replication. One protein, 2'5'-oligo-adenylate synthetase, indirectly activates an endoribonuclease that degrades viral RNA. Another protein, p68 serine/threonine protein kinase (also known as PKR and TIK), is induced following interferon stimulation and activated by autophosphorylation in the presence of dsRNA. Upon activation, p68 phosphorylates the alpha subunit of the eukaryotic initiation factor 2 (eIF-2) resulting in inhibition of protein synthesis and, in turn, inhibition of viral replication. Evidence also suggests that p68 protein kinase inhibits proliferation and potentiates tumor suppressor function. In addition, p68 has been shown to phosphorylate Ikappa-B, thus activating NF-kappaB which induces interferon-beta gene transcription. This antibody is routinely tested by western blot analysis.
Synonyms: Protein Kinase R, p68 Kinase, TIK
|途径||ER-Nucleus Signaling, Hepatitis C|
使用细节EIF2AK2抗体详情 EIF2AK2目标详情 贮存及处理 References for anti-EIF2AK2 antibody (ABIN968165) 图像 回页首
Related Products: ABIN968533, ABIN967389
贮存及处理EIF2AK2抗体详情 EIF2AK2目标详情 使用细节 References for anti-EIF2AK2 antibody (ABIN968165) 图像 回页首
|缓冲液||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|注意事项||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|储存方法||Store undiluted at -20° C.|
References for anti-EIF2AK2 antibody (ABIN968165)EIF2AK2抗体详情 EIF2AK2目标详情 使用细节 贮存及处理 图像 回页首
Feng, Chong, Kumar, Williams: "Identification of double-stranded RNA-binding domains in the interferon-induced double-stranded RNA-activated p68 kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5447-51, 1992
Lee, Tomita, Hovanessian, Katze: "Characterization and regulation of the 58,000-dalton cellular inhibitor of the interferon-induced, dsRNA-activated protein kinase." in: The Journal of biological chemistry, Vol. 267, Issue 20, pp. 14238-43, 1992
Barber, Tomita, Hovanessian, Meurs, Katze: "Functional expression and characterization of the interferon-induced double-stranded RNA activated P68 protein kinase from Escherichia coli." in: Biochemistry, Vol. 30, Issue 42, pp. 10356-61, 1991
Meurs, Chong, Galabru, Thomas, Kerr, Williams, Hovanessian: "Molecular cloning and characterization of the human double-stranded RNA-activated protein kinase induced by interferon." in: Cell, Vol. 62, Issue 2, pp. 379-90, 1990
图像EIF2AK2抗体详情 EIF2AK2目标详情 使用细节 贮存及处理 References for anti-EIF2AK2 antibody (ABIN968165) 回页首