We are preparing the requested document. Please wait, this may take a while...!
|抗原||Polynucleotide Kinase 3'-Phosphatase (PNKP) 抗体|
|抗原表位||Internal Region 其他选择|
|适用||Chimpanzee, 人 其他选择|
|标记||This PNKP antibody is un-conjugated 其他选择|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|Supplier||Log in to see|
PNKP抗体详情PNKP目标详情 使用细节 贮存及处理 图像
|特异性||This affinity purified antibody is directed against PNK protein.|
Species reactivity (expected):Chimpanzee (100 %).Dog, mouse and rat (83 %).
Species reactivity (tested):Human.
|产品特性||Concentration: 1.46 mg/mL (by UV absorbance at 280 nm)|
|免疫原||Synthetic peptide corresponding to an internal region of human PNK protein|
|Plasmids, Primers & others|
PNKP目标详情PNKP抗体详情 使用细节 贮存及处理 图像 回页首
|别名||PNKP (PNKP Antibody 摘要)|
|背景||Mammalian PNK (also known as PNKP, Polynucleotide kinase 3'-phosphatase, DNA 5'-kinase/3'- hosphatase and Polynucleotide kinase) catalyzes the 5' phosphorylation of nucleic acids and can have associated 3' phosphatase activity, predictive of an important function in DNA repair following ionizing radiation or oxidative damage. PNK is a nuclear protein that is expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine.Synonyms: Bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase-3'-phosphatase|
|途径||DNA Damage Repair, Nucleotide Phosphorylation|
使用细节PNKP抗体详情 PNKP目标详情 贮存及处理 图像 回页首
ELISA: 1: 3,000 - 1: 12,000. Western blot: 1: 300 - 1: 1,500 (Expect a band approximately 57 kDa in size corresponding toPNK in the appropriate cell lysate or extract).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
贮存及处理PNKP抗体详情 PNKP目标详情 使用细节 图像 回页首
|缓冲液||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide|
|注意事项||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|注意事项||Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.|
|储存条件||4 °C/-20 °C|
|储存方法||Store the antibody at 2 - 8 °C up to one month or for longer (in aliquots) at -20 °C or below.|