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|抗原||PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2) 抗体|
|抗原表位||AA 58-81 其他选择|
|适用||Chimpanzee, Hamster, 人, 小鼠, 大鼠 其他选择|
|标记||This PMS2 antibody is un-conjugated 其他选择|
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
|Supplier||Log in to see|
PMS2抗体详情PMS2目标详情 使用细节 贮存及处理 图像
|特异性||This antibody is directed against PMS2-134 and reacts with full length version of PMS2. The epitope was putatively mapped to amino acids 58-81 of human PMS2. Expect a band approximately 96 kDa in size corresponding to human PMS2 by western blotting in most cell lines and tissues as PMS2 is ubiquitously expressed.|
|交叉反应 （详细）||Species reactivity (expected):Mouse, Rat, Chimpanzee (100 %)Species reactivity (tested):Human, Hamster|
Synonyms: PMSL2, Mismatch repair endonuclease PMS2, PMS1 protein homolog 2
Concentration: 1.38 mg/mL (by UV absorbance at 280 nm)
|免疫原||Recombinant human PMS2 corresponding to the first 133 amino acid residues of theprotein|
|Plasmids, Primers & others|
PMS2目标详情PMS2抗体详情 使用细节 贮存及处理 图像 回页首
|别名||PMS2 (PMS2 Antibody 摘要)|
|背景||PMS2 is a highly conserved nuclear protein involved in mismatch repair during DNA replication and has been identified to be composed as a heterodimer of PMS2 and MLH1. PMS is part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4), Turcot syndrome (an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas) and supratentorial primitive neuroectodermal tumors with cafe-au-lait spots (SNTCL). The human PMS2 gene encodes an 862 aa, 96 kDa polypeptide.Synonyms: DNA mismatch repair protein PMS2, Mismatch repair endonuclease PMS2, PMS1 protein homolog 2, PMSL2|
|途径||DNA Damage Repair, Production of Molecular Mediator of Immune Response|
使用细节PMS2抗体详情 PMS2目标详情 贮存及处理 图像 回页首
ELISA: 1: 5,000 - 1: 20,000. Western blot: 1: 500 - 1: 2,000. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
贮存及处理PMS2抗体详情 PMS2目标详情 使用细节 图像 回页首
|缓冲液||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 a buffer and 0.09 % (w/v) Sodium Azide as preservative|
|注意事项||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|储存条件||4 °C/-20 °C|
Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C forlonger. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Shelf life: one year from despatch.