anti-人 PMS2 抗体 for Immunohistochemistry (Paraffin-embedded Sections)

Recommended PMS2 Antibody (supplied by: Log in to see )

抗原
PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2) 抗体
  • HNPCC4
  • PMS2CL
  • PMSL2
  • AW555130
  • Pmsl2
  • PMS1 homolog 2, mismatch repair system component
  • PMS1 homolog2, mismatch repair system component
  • mismatch repair endonuclease PMS2
  • PMS2
  • Pms2
  • LOC463257
  • LOC107984056
抗原表位
C-Term
9
8
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
适用

125
42
19
2
2
2
2
1
1
1
1
1
1
1
1
宿主
小鼠
72
53
1
克隆类型
单克隆
标记
This PMS2 antibody is un-conjugated
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
99
55
53
34
28
19
12
9
3
2
2
1
选项
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产品编号 ABIN1027499
$ 1,076.44
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
15.811565 ABIN1027500 IHC (p) Mouse IgG1 kappa C-Term Log in to see A16-4 0
11.7556305 ABIN241578 IHC IHC (p) WB Mouse IgG1 AA 623-639 Log in to see 163C1251 0
9.741807 ABIN4346521 IF IHC IHC (p) WB Mouse IgG2b Log in to see 2G5 1
8.7556305 ABIN5611553 EIA IHC (p) Rabbit IgG C-Term Log in to see Polyclonal 0
7 ABIN5585870 IHC (p) WB Mouse IgG1 Log in to see 163C1251 0
4 ABIN2878780 ELISA IHC IHC (p) Rabbit IgG AA 125-174 Log in to see Polyclonal 0
4 ABIN400707 IHC (p) WB Mouse IgG1 AA 623-639 Log in to see 163C1251 0
4 ABIN1723117 IHC (p) WB Mouse IgG1 AA 623-639 Log in to see 163C1251 0
4 ABIN5960584 ELISA IF IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
4 ABIN317683 IF IHC (p) WB Rabbit Log in to see Polyclonal 0
4 ABIN1738171 IHC (p) ELISA Rabbit IgG Log in to see Polyclonal 0
3.9974375 ABIN5951374 FACS ICC IF IHC IHC (p) IP WB Rabbit IgG Log in to see SY08-09 0
1 ABIN127133 IHC (p) IP WB Rabbit Log in to see Polyclonal 0
1 ABIN2581152 IHC IHC (p) WB Mouse IgG2b Log in to see 4B7 0
1 ABIN2581153 IHC IHC (p) WB Mouse IgG2b Log in to see 2G5 0
1 ABIN2770550 IHC (p) WB Mouse IgG1 AA 623-639 Log in to see 163C1251 0
1 ABIN2764352 ELISA IHC (p) Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN2729222 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
-2.0025625 ABIN252510 CyTOF FACS IHC IHC (p) WB Mouse IgG1 Log in to see 163C1251 0
-12.758193 ABIN4346504 CyTOF FACS IHC IHC (p) WB Mouse IgG1 Log in to see 163C1251 0

General

抗原 PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2) 抗体
抗原表位 C-Term
(9), (8), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
适用
(125), (42), (19), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
宿主 小鼠
(72), (53), (1)
克隆类型 (克隆位点)
单克隆   ( )
标记 This PMS2 antibody is un-conjugated
(3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(99), (55), (53), (34), (28), (19), (12), (9), (3), (2), (2), (1)
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PMS2抗体详情

PMS2目标详情 使用细节 贮存及处理 图像
原理 This antibody is designed for the specific localization of human PMS2 using IHC techniques in formalin-fixed, paraffin- embedded tissue sections.
产品特性 PMS2 belongs to the mismatch repair proteins (MMR proteins) like MLH1, MLH3, MSH2, MSH3, MSH4, MSH5, MSH6, and PMS1. The PMS2 gene is homologue to the prokaryontic MutL gene. Defects in the genes coding for MMR proteins lead to microsatellite instability (MSI) and considerable higher mutation rates. Hereditary nonpolyposis colorectal cancers (HNPCC) often show germline mutations in the mmr protein associated genes. These mutations result in decreased or abnormal protein production. The majority of HNPCC is characterised by damages in the MLH1 and MSH2 encoding genes, sometimes also in damages in MSH6 and PMS2 encoding genes. Immunohistochemistry (IHC) is a complex technique in which immunological and histological detection methods are combined. In general, the manipulation and processing of tissues before immunostaining, especially different types of tissue fixation and embedding, as well as the nature of the tissues themselves may cause inconsistent results (Nadji and Morales, 1983). Endogenous pseudoperoxidase and peroxidase activity or endogenous biotin and alkaline phosphatase activity can cause non-specific staining results depending on the detection system used. Tissues that contain Hepatitis B surface antigen (HBsAg) can produce false positives when using HRP detection systems (Omata et al, 1980). Insufficient contrast staining and/or improper mounting of the sample may influence the interpretation of results.
纯化方法 Ready-to-use, Prediluted,
试剂未包括 All reagents, materials, and laboratory equipment for IHC procedures are not provided with this antibody. This includes adhesive slides and cover slips, positive and negative control tissues, Xylene or adequate substitute, ethanol, distilled H2O, heat pretreatment equipment (pressure cooker, steamer, microwave), pipettes, Coplin jars, glass jars, moist chamber, histological baths, negative control reagents, counter-staining solution, mounting materials, and microscope.
免疫原 Recombinant human PMS2 (C-Terminus).
克隆位点 A16-4
亚型 IgG1 kappa
Plasmids, Primers & others

PMS2目标详情

PMS2抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 PMS2 (PMS2 Antibody 摘要)
途径 DNA Damage Repair, Production of Molecular Mediator of Immune Response

使用细节

PMS2抗体详情 PMS2目标详情 贮存及处理 图像 回页首
说明

Staining pattern: Nuclear. The interpretation of the stain results is the full responsibility of the user. Any experimental result must be confirmed by a medically established diagnostic product or procedure.
Positive control: Tissue sample from placenta or colon adenocarcinoma.
External Negative Control: Tissue sample homologous to the test sample incubated with an antibody isotype not specific for PMS2.

实验流程 Principles of the procedure: The demonstrations of antigens by IHC is a sequential procedure with several steps involving first the application of a specific antibody for the antigen of interest (primary antibody), then a secondary antibody which joins to the first, an enzyme complex, and the addition of a chromogenic substrate. The sample is washed between each step. Enzymatic activation of the chromogenic substrate creates a visible product where the antigen is located. The results are interpreted using a light microscope. The primary antibody can be used both in manual IHC and with automated immunostainers.

Paraffin-embedded tissue samples should be used. Western blot techniques are not recommended.
实验流程 Antigen retrieval: HIER Citrate Buffer pH 6.5
Working dilution: (only for concentrates) 1:25 – 1:100
Incubation: 30 min, RT
Control Tissue: Placenta, colon adenocarcinoma
限制 仅限研究用

贮存及处理

PMS2抗体详情 PMS2目标详情 使用细节 图像 回页首
状态 Liquid
缓冲液 The preparation contains saline buffer, stabilising and carriers proteins. (pH 7.4)
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Store at 2-8 °C until the expiration date printed on product label. Do not use after the expiration date. If fresh solutions are required, these must be prepared immediately prior to use, and will be stable for at least one day at room temperature (20-25°C). Unused portion of antibody preparation should be discarded after one day.If the product is stored under different conditions from those stipulated in these technical indications, the new conditions must be verified by the user. The validity period of the ready to use products when opened, is the same as the expiration date indicated on the label of intact product.

图像

PMS2抗体详情 PMS2目标详情 使用细节 贮存及处理 回页首
厂商提供的图像
Immunohistochemistry (IHC) image for anti-PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2) (C-Term) antibody (ABIN1027499) 10 × 20 × IHC of PMS2 (clone A16-4) on FFPE colon carcinoma tissue. Citrate pH 6.5, D...