anti-大鼠 PALB2 抗体 for Immunofluorescence (Paraffin-embedded Sections)

Recommended PALB2 Antibody (supplied by: Log in to see )

抗原
Partner and Localizer of BRCA2 (PALB2) 抗体
  • PALB2
  • Fancn
  • si:ch211-14k19.9
  • FANCN
  • PNCA3
  • 4732427B05
  • BC066140
  • RGD1304759
  • partner and localizer of BRCA2
  • partner and localizer of BRCA2 L homeolog
  • PALB2
  • palb2
  • palb2.L
  • Palb2
抗原表位
AA 1100-1150
9
8
8
7
7
3
2
2
1
1
1
1
适用
人, 小鼠, 大鼠
66
15
12
宿主

49
17
克隆类型
多克隆
标记
This PALB2 antibody is un-conjugated
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
59
45
27
17
7
5
4
2
2
选项
Supplier
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Supplier Product No.
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Available images

产品编号 ABIN670536
$ 306.90
加上运输费 $45.00

General

抗原 Partner and Localizer of BRCA2 (PALB2) 抗体
抗原表位 AA 1100-1150
(9), (8), (8), (7), (7), (3), (2), (2), (1), (1), (1), (1)
适用 人, 小鼠, 大鼠
(66), (15), (12)
宿主
(49), (17)
克隆类型
多克隆
标记 This PALB2 antibody is un-conjugated
(4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(59), (45), (27), (17), (7), (5), (4), (2), (2)
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PALB2抗体详情

PALB2目标详情 使用细节 贮存及处理 图像
纯化方法 Purified by Protein A.
免疫原 KLH conjugated synthetic peptide derived from human PALB2
亚型 IgG
Plasmids, Primers & others

PALB2目标详情

PALB2抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 PALB2 (PALB2 Antibody 摘要)
背景

Plays a critical role in homologous recombination repair (HRR) through its ability to recruit BRCA2 and RAD51 to DNA breaks. Strongly stimulates the DNA strand-invasion activity of RAD51, stabilizes the nucleoprotein filament against a disruptive BRC3-BRC4 polypeptide and helps RAD51 to overcome the suppressive effect of replication protein A (RPA). Functionally cooperates with RAD51AP1 in promoting of D-loop formation by RAD51. Serves as the molecular scaffold in the formation of the BRCA1-PALB2-BRCA2 complex which is essential for homologous recombination. Via its WD repeats is proposed to scaffold a HR complex containing RAD51C and BRCA2 which is thought to play a role in HR-mediated DNA repair. Essential partner of BRCA2 that promotes the localization and stability of BRCA2. Also enables its recombinational repair and checkpoint functions of BRCA2. May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteasome-mediated degradation. Binds DNA with high affinity for D loop, which comprises single-stranded, double-stranded and branched DNA structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks, together with BRCA2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity.

Subcellular location: Nucleus

Synonyms: FANCN, PNCA3, Partner and localizer of BRCA2, PALB2

基因ID 79728
UniProt Q86YC2
途径 DNA Damage Repair

使用细节

PALB2抗体详情 PALB2目标详情 贮存及处理 图像 回页首
应用备注 WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
限制 仅限研究用

贮存及处理

PALB2抗体详情 PALB2目标详情 使用细节 图像 回页首
状态 Liquid
浓度 1 μg/μL
缓冲液 Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Store at -20°C
有效期 12 months

图像

PALB2抗体详情 PALB2目标详情 使用细节 贮存及处理 回页首
厂商提供的图像
Immunohistochemistry (IHC) image for anti-Partner and Localizer of BRCA2 (PALB2) (AA 1100-1150) antibody (ABIN670536) Formalin-fixed and paraffin embedded human breast carcinoma labeled with Rabbit Anti ...
Western Blotting (WB) image for anti-Partner and Localizer of BRCA2 (PALB2) (AA 1100-1150) antibody (ABIN670536) Images provided by the Independent Validation Program (badge number 29749). Lane 1: A...
Flow Cytometry (FACS) image for anti-Partner and Localizer of BRCA2 (PALB2) (AA 1100-1150) antibody (ABIN670536) HeLa cells probed with PALB2 Polyclonal Antibody, Unconjugated at 1:100 for 30 minut...
'独立验证'标志
Lot Number 120510
Method validated Western Blotting
Positive Control A549 cell extract
Negative Control c6/36 mosquito cell extract (non-reactive species)
Notes A single strong band was observed in the positive control at the correct molecular weight. Several faint bands appeared in the negative control, but they were much less intense than the positive control band.
Primary Antibody
  • Antigen: Partner and Localizer of BRCA2 (PALB2) (1:500 dilution)
  • Catalog number: ABIN670536
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 120510
Secondary Antibody
  • Antibody: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:20,000 dilution)
  • Supplier: Log in to see
  • Catalog number: Log in to see
  • Lot number: L170-6515
Controls
  • Positive control: A549 cell extract
  • Negative control: c6/36 Mosquito cell extract
实验流程
  • 1. Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
  • 2. 35 μg of boiled extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • 5. The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 h.
  • 6. The membrane was rinsed with TBST once.
  • 7. The membrane was immersed with the protein side up in the primary antibody solution (anti-PALB2; 1:500) in TBST containing 5% (W/V) BSA and incubated for 16 h at 4°C.
  • 8. The membrane was rinsed in TBST thrice for 5 min each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) BSA and incubated for 1 h at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice TBST thrice for 5 min each.
  • 11. The membrane was rinsed in TBS twice for 30 s each.
  • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
  • 13. The membrane was rinsed three times TBST.
  • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
  • 15. The membrane was washed in TBST 2 times for 10 min each.
  • 16. Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).
Experimental Notes - No challenges noted.
Validation Images
Western Blotting validation image for anti-Partner and Localizer of BRCA2 (PALB2) (AA 1100-1150) antibody (ABIN670536) Figure 1. Western blot of lysates from A549 cells (Lane 1) and c6/36 cells (Lane 2) p...