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|抗原||8-Oxoguanine DNA Glycosylase (OGG1) 抗体|
|适用||人, 小鼠, 灵长类, 大鼠 其他选择|
|标记||This OGG1 antibody is un-conjugated 其他选择|
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
|Supplier||Log in to see|
OGG1抗体详情OGG1目标详情 使用细节 贮存及处理 References for anti-OGG1 antibody (ABIN151034) 图像
|纯化方法||Immunogen affinity purified|
|免疫原||A peptide derived from human Ogg1 (within amino acids 1-100). [UniProt# O15527]|
|Plasmids, Primers & others|
OGG1目标详情OGG1抗体详情 使用细节 贮存及处理 References for anti-OGG1 antibody (ABIN151034) 图像 回页首
|别名||OGG1 (OGG1 Antibody 摘要)|
|背景||Gene Symbol: OGG1|
|分子量||Theoretical MW: 39 kDa|
|途径||DNA Damage Repair|
使用细节OGG1抗体详情 OGG1目标详情 贮存及处理 References for anti-OGG1 antibody (ABIN151034) 图像 回页首
|应用备注||Western Blot 1:500-1:1000, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:100 - 1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Frozen 1:25-1:100This Ogg1 antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation Co-IP (PMID: 20956573), ELISA (PMID: 19506022) and Western Blot. In WB, it recognizes a band at ~39 kDa, representing Ogg1. In ICC/IF nuclear staining was observed in Hek293 cells. Flow data from customer review. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Western blot Protocol for Ogg1 Antibody Western Blot Procedure
1. Run 50 µg of protein on a 4-20 % Tris-glycine mini-gel at 125V for 90 minutes.
. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes.
. Transfer protein to the membrane at 25V for 90 minutes.
. Allow membrane to air-dry.
. Block membrane with 1XPBS/3 % BSA for 1 hour at room temperature (23-27 degrees C).
. Wash membrane twice, for 5 minutes each, with 1XPBS/0.05 % Tween-20 (PBST).
. Incubate membrane with NB100-106 (anti-hOGG1), diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Incubate membrane with goat anti-rabbit IgG-HRP, diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Detect cross-reacting proteins using Renaissance Chemiluminescence Reagent Plus kit from NEN Life Sciences.Immunohistochemistry Protocol for Ogg1 Antibody Immunohistochemistry - FFPE sectionsI. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution:Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol.Use within 4 hours of preparationB. Place slides in distilled water: 2 changes for 2 minutes each.III. Retrieve Epitopes:A. Preheat Citrate
贮存及处理OGG1抗体详情 OGG1目标详情 使用细节 References for anti-OGG1 antibody (ABIN151034) 图像 回页首
Buffer contains: 0.05 % Sodium Azide
|注意事项||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|注意事项||Avoid freeze-thaw cycles|
|储存条件||-80 °C,-20 °C|
|储存方法||Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.|
References for anti-OGG1 antibody (ABIN151034)OGG1抗体详情 OGG1目标详情 使用细节 贮存及处理 图像 回页首
Wang, Lai, Li, Chiang, Yu, Sakamoto, Tu, Hsieh, Huang, Chuang, Dai, Yu: "The Paradoxical Effects of Different Hepatitis C Viral Loads on Host DNA Damage and Repair Abilities." in: PLoS ONE, Vol. 12, Issue 1, pp. e0164281, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).
Pillai, Kanwal, Fang, Sharp, Samant, Arbiser, Gupta: "Honokiol, an activator of Sirtuin-3 (SIRT3) preserves mitochondria and protects the heart from doxorubicin-induced cardiomyopathy in mice." in: Oncotarget, Vol. 8, Issue 21, pp. 34082-34098, 2017 Method employed by authors: Immunoprecipitation (IP) (Sample species: Mouse (Murine)).
Martin, Wong: "Enforced DNA repair enzymes rescue neurons from apoptosis induced by target deprivation and axotomy in mouse models of neurodegeneration." in: Mechanisms of ageing and development, Vol. 161, Issue Pt A, pp. 149-162, 2017
Moreli, Santos, Lorenzon-Ojea, Corrêa-Silva, Fortunato, Rocha, Rudge, Damasceno, Bevilacqua, Calderon: "Hyperglycemia Differentially Affects Maternal and Fetal DNA Integrity and DNA Damage Response." in: International journal of biological sciences, Vol. 12, Issue 4, pp. 466-77, 2016
Liśkiewicz, Kasprowska, Wojakowska, Polański, Lewin-Kowalik, Kotulska, Jędrzejowska-Szypułka: "Long-term High Fat Ketogenic Diet Promotes Renal Tumor Growth in a Rat Model of Tuberous Sclerosis." in: Scientific reports, Vol. 6, pp. 21807, 2016 Method employed by authors: Immunohistochemistry (IHC)
Leon, Sakumi, Castillo, Sheng, Oka, Nakabeppu: "Corrigendum: 8-Oxoguanine accumulation in mitochondrial DNA causes mitochondrial dysfunction and impairs neuritogenesis in cultured adult mouse cortical neurons under oxidative conditions." in: Scientific reports, Vol. 6, pp. 24696, 2016 Method employed by authors: Immunohistochemistry (IHC)
Leon, Sakumi, Castillo, Sheng, Oka, Nakabeppu: "8-Oxoguanine accumulation in mitochondrial DNA causes mitochondrial dysfunction and impairs neuritogenesis in cultured adult mouse cortical neurons under oxidative conditions." in: Scientific reports, Vol. 6, pp. 22086, 2016 Method employed by authors: Western Blotting (WB)
Kohno, Yamamoto, Hirahashi, Kumagae, Nakamura, Oki, Oda: "Reduced MUTYH, MTH1, and OGG1 expression and TP53 mutation in diffuse-type adenocarcinoma of gastric cardia." in: Human pathology, Vol. 52, pp. 145-52, 2016 Method employed by authors: Western Blotting (WB)
Chaisiriwong, Wanitphakdeedecha, Sitthinamsuwan, Sampattavanich, Chatsiricharoenkul, Manuskiatti, Panich: "A Case-Control Study of Involvement of Oxidative DNA Damage and Alteration of Antioxidant Defense System in Patients with Basal Cell Carcinoma: Modulation by Tumor Removal." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 5934024, 2016
Tarry-Adkins, Fernandez-Twinn, Chen, Hargreaves, Neergheen, Aiken, Ozanne: "Poor maternal nutrition and accelerated postnatal growth induces an accelerated aging phenotype and oxidative stress in skeletal muscle of male rats." in: Disease models & mechanisms, Vol. 9, Issue 10, pp. 1221-1229, 2016
Yang, Chen, Chen, Kuo, Chen: "Activation of GLP-1 Receptor Enhances Neuronal Base Excision Repair via PI3K-AKT-Induced Expression of Apurinic/Apyrimidinic Endonuclease 1." in: Theranostics, Vol. 6, Issue 12, pp. 2015-2027, 2016
Nikitaki, Nikolov, Mavragani, Mladenov, Mangelis, Laskaratou, Fragkoulis, Hellweg, Martin, Emfietzoglou, Hatzi, Terzoudi, Iliakis, Georgakilas: "Measurement of complex DNA damage induction and repair in human cellular systems after exposure to ionizing radiations of varying linear energy transfer (LET)." in: Free radical research, Vol. 50, Issue sup1, pp. S64-S78, 2016 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).
Fridlich, Annamalai, Roy, Bernheim, Powell: "BRCA1 and BRCA2 protect against oxidative DNA damage converted into double-strand breaks during DNA replication." in: DNA repair, Vol. 30, pp. 11-20, 2015 (Sample species: Human). Further details: Western Blotting
Zijno, De Angelis, De Berardis, Andreoli, Russo, Pietraforte, Scorza, Degan, Ponti, Rossi, Barone: "Different mechanisms are involved in oxidative DNA damage and genotoxicity induction by ZnO and TiO2 nanoparticles in human colon carcinoma cells." in: Toxicology in vitro : an international journal published in association with BIBRA, Vol. 29, Issue 7, pp. 1503-12, 2015 (Sample species: Human). Further details: Western Blotting
Dziaman, Banaszkiewicz, Roszkowski, Gackowski, Wisniewska, Rozalski, Foksinski, Siomek, Speina, Winczura, Marszalek, Tudek, Olinski: "8-Oxo-7,8-dihydroguanine and uric acid as efficient predictors of survival in colon cancer patients." in: International journal of cancer, Vol. 134, Issue 2, pp. 376-83, 2014 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Cordero, Alcocer-Gómez, Culic, Carrión, de Miguel, Díaz-Parrado, Pérez-Villegas, Bullón, Battino, Sánchez-Alcazar: "NLRP3 inflammasome is activated in fibromyalgia: the effect of coenzyme Q10." in: Antioxidants & redox signaling, Vol. 20, Issue 8, pp. 1169-80, 2014
Kubo, Morita, Nakashima, Kitao, Egashira, Saeki, Oki, Kakeji, Oda, Maehara: "Oxidative DNA damage in human esophageal cancer: clinicopathological analysis of 8-hydroxydeoxyguanosine and its repair enzyme." in: Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus, Vol. 27, Issue 3, pp. 285-93, 2014 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Xu, Wang, Guo, Zhou, Lv, Chen, Liu: "The significance of the alteration of 8-OHdG in serous ovarian carcinoma." in: Journal of ovarian research, Vol. 6, Issue 1, pp. 74, 2014
Kim, Cheresh, Williams, Cheng, Ridge, Schumacker, Weitzman, Bohr, Kamp: "Mitochondria-targeted Ogg1 and aconitase-2 prevent oxidant-induced mitochondrial DNA damage in alveolar epithelial cells." in: The Journal of biological chemistry, Vol. 289, Issue 9, pp. 6165-76, 2014 (Sample species: Human). Further details: Western Blotting
Kumar, Rao, Pal, Pal: "Hyperglycemia-induced oxidative stress induces apoptosis by inhibiting PI3-kinase/Akt and ERK1/2 MAPK mediated signaling pathway causing downregulation of 8-oxoG-DNA glycosylase levels in glial cells." in: The international journal of biochemistry & cell biology, Vol. 53, pp. 302-19, 2014 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence
图像OGG1抗体详情 OGG1目标详情 使用细节 贮存及处理 References for anti-OGG1 antibody (ABIN151034) 回页首