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Human MCM2 Protein expressed in Wheat germ - ABIN1310547
Henderson, Hall, Prpic, Hessling, Parker, Sampson, Simkins, Brough, Dixon, Lenz, Knapp, Murphy, Taylor, Fischer, Malinowski: The selection and characterization of antibodies to minichromosome maintenance proteins that highlight cervical dysplasia. in Journal of immunological methods 2011
Data show the structure of the Cdc45/Mcm2-7/Psf1 GINS (CMG) helicase in the presence of ATPgammaS and a DNA duplex bearing a 3' single-stranded tail.
the foxn1-mcm2 axis plays a central role in the genetic regulatory network controlling thymus development in zebrafish
The Cox regression model revealed that only NIP7, RPL10L, and MCM2 exhibited significant correlation with distant recurrence-free survival in liposarcoma in the GSE30929 dataset, and the regression coefficients were -0.676, -0.703, and 0.868, respectively
Minichromosome maintenance proteins (MCM), a eukaryotic DNA helices complex required for the process of DNA replication, are loaded onto chromatin during the G1 phase and define potential locations of DNA replication initiation.
The findings suggest that Mcm-2 may be a sensitive proliferation marker in oral potentially malignant and malignant lesions which may be useful for differentiating between Verrucous Hyperplasia (VH) with/ without dysplasia, Verrucous Carcinoma (VC) and Oral Squamous Cell Carcinoma (OSCC).
High MCM2 expression is associated with HIV-1 infection.
The results showed that MUS81 modulates MCM2 levels as well as homologous recombination (HR) activity. Moreover, downregulation of MUS81 increased the sensitivity of epithelial ovarian cancer (EOC)cells to olaparib by inducing S phase arrest and promoting apoptosis through activation of MCM2. MUS81 may be a potential novel therapeutic target for EOC.
MCM2 played important roles in regulating cell cycle- and DNA replication-related pathways. E2F could upregulate the expression levels of MCM2 by deregulating the methylations of their promoters to promote the relapse of Acute Lymphoblastic Leukemia .
ProEx C is an immunohistochemical cocktail containing antibodies direct against topoisomerase IIalpha (TOP2A) and minichromosome maintenance 2 (MCM2) proteins. This brief review covers the effective utility of ProEx C as adjunct tool in assessing the urothelial lesions in urine cytology, also providing prognostic and therapeutic information to help in clinical decisions.
the decreased growth of osteosarcoma cells by MCM2 or MCM3 knockdown was reversed by DHX9 overexpression, indicating that MCM2 and MCM3 activity was DHX9-dependent.
Results show that MCM2 is significantly upregulated in urothelial carcinoma.
Sld5 a component of GINS complex interacts with SIK1 and recruits it to the sites of DNA replication at the onset of S phase.
MCM2 expression in serrated colonic polyps demonstrates aberrant cellular proliferation and changes in the colonic microenvironment may promote adenoma morphogenesis and predisposition to malignancy.
these findings suggest that lnc-FTX may act as a tumor suppressor in hepatocellular carcinoma (HCC) through physically binding miR-374a and MCM2. It may also be one of the reasons for HCC gender disparity and may potentially contribute to HCC treatment
Data suggest that interaction of Mcm10 with Mcm2-7 multimer requires Mcm10 domain that contains amino acids 530-655, which overlaps with domain required for stable retention of Mcm10 on chromatin; Mcm10 conserved domain (amino acids 200-482) is essential for DNA replication; both conserved domain and Mcm2-7-binding domain are required for full activity of Mcm10.
MCM2-MCM6 complex is required for CHK2 chromatin loading and its phosphorylation to DNA damage response in squamous cell carcinoma cells.
BD ProExtrade mark C assay containing MCM2 and TOP2A antibodies showed strong specific nuclear staining that correlated with increased cervical dysplasia and lesion severity.
these data suggest that positive MCM2 and negative TIP30 expression are closely correlated with the clinical, pathological and biological parameters, in addition to poor prognosis in patients with gallbladder cancer.
knockdown of MCM2 or MCM6 could significantly inhibit foci forming of MDC1 in TE-1 nuclei in response to bleomycin-induced DNA damage (p < 0.001). This study indicates the direct interaction between MDC1 and MCMs in TE-1 nuclei.
PTEN regulates DNA replication through MCM2 and loss of PTEN function leads to replication defects and genomic instability.
MCM2 expression is higher in ovarian and endometrial high-grade serous carcinomas (HGSC) than in ovarian or endometrial endometrioid carcinoma. Knockdown in ovarian HGSC cell line decreased cell proliferation.
quaternary complex of histone H3-H4 heterodimer with chaperone ASF1 and the replicative helicase subunit MCM2
Data show that MRE11- and RAD51-dependent fork repair leading to reloading of the GINS onto the MCM-CDC45 complex still engaged with the DNA could be sufficient to restore a functional CDC45-MCM-GINS (CMG) helicase complex.
Mcm2-7 associate with Cdc45 and GINS to form a relatively stable CMG (Cdc45-MCM-GINS) complex.
ATM and ATR phosphorylate the functionally critical replication protein Mcm2 during both DNA damage and replication checkpoint responses in Xenopus egg extracts
MCM2-7 is the replicative helicase, dispensable for chromatin loading and pre-replicative complex (pre-RC) assembly, but required for origin unwinding during DNA replication
Segregation of parental histones to the leading strand increased markedly in cells with histone-binding mutations in MCM2, part of the replicative helicase, exacerbating histone posttranslational modifications sister chromatid asymmetry.
MCM2 is dynamically expressed in both proliferative and differentiated stromal cells during mouse periimplantation uterus. expression of Mcm2 is induced by progesterone action in the mouse uterine stroma. siRNA-mediated silencing of MCM2 arrests the cell cycle at G1-S transition during stromal cell proliferation. downregulation of Mcm2 could also compromise stromal cell differentiation.
MCM2 deficiency reduces DNA replication initiation in gene-rich regions of the genome.
these data demonstrate that active MCM2-7 repression is a physiologically important mechanism for RS-induced cell cycle arrest and genome maintenance on an organismal level.
Hematopoietic cells originally express higher levels MCM 2 protein and undergo more frequent apoptosis following doxorubicin-induced DNA-damage in the presence of the FLV envelope protein gp70.
Mcm2 deficiency results in short deletions allowing high resolution identification of genes contributing to lymphoblastic lymphoma.
Accumulation and dynamics of Mcm2 are described during oogenesis and the first embryonic cell cycle.
gp70 enhances cellular DNA-damage-induced signaling in association with host-specific cellular proteins including acinus and MCM2 resulting in the activation of DNA-PK to phosphorylate P53.
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
, minichromosome maintenance 2
, DNA replication licensing factor mcm2
, MCM2 minichromosome maintenance deficient 2, mitotin
, minichromosome maintenance protein 2
, DNA replication licensing factor MCM2
, cell devision cycle-like 1
, cyclin-like 1
, minichromosome maintenance deficient 2 (mitotin)
, minichromosome maintenance protein 2 homolog
, nuclear protein BM28
, minichromosome maintenance deficient 2 mitotin
, minichromosome maintenance complex component 7
, mini chromosome maintenance deficient 2