anti-大鼠 Complement Factor H 抗体 for Immunohistochemistry

Recommended Complement Factor H Antibody

抗原
Complement Factor H (CFH) 抗体
  • AHUS1
  • AMBP1
  • ARMD4
  • ARMS1
  • CFHL3
  • FH
  • FHL1
  • HF
  • HF1
  • HF2
  • HUS
  • Mud-1
  • NOM
  • Sas-1
  • Sas1
  • Fh
  • CFH
  • im:7147412
  • si:ch211-207o17.2
  • fH
  • armd4
  • arms1
  • cfh
  • cfhl3
  • fhl1
  • hf1
  • hf2
  • hus
  • complement factor H
  • complement component factor h
  • complement factor H-related protein 5
  • complement factor H L homeolog
  • CFH
  • Cfh
  • cfh
  • LOC100399888
  • LOC100733902
  • cfh.L
适用
小鼠, 大鼠, 人
231
34
33
3
3
3
3
2
2
宿主

121
85
32
16
1
1
克隆类型
多克隆
标记
This Complement Factor H antibody is un-conjugated
19
12
5
4
4
4
4
4
4
4
4
3
2
2
2
2
2
1
应用范围
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
166
118
75
42
33
26
23
22
15
10
10
9
9
5
4
1
1
1
1
选项

Available images

产品编号 ABIN3023097
$ 393.25
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
19.082928 ABIN1858382 ICC IHC WB Rabbit IgG AA 975-1217 Polyclonal 0
16 ABIN2896779 IF/ICC IHC IP WB Rabbit AA 975-1217 Polyclonal 0
13.082929 ABIN6260777 ELISA IHC WB Rabbit IgG Polyclonal 0
7 ABIN6002151 IF/ICC IHC IP WB Mouse AA 975-1217 0
7 ABIN6002153 IF/ICC IHC IP WB Mouse AA 975-1217 0
7 ABIN6002155 IF/ICC IHC IP WB Mouse AA 975-1217 0
4 ABIN6673510 IF IHC WB Rabbit IgG Polyclonal 0
1 ABIN2878086 IHC WB Rabbit Polyclonal 0
1 ABIN6055723 IF/ICC IHC WB Biotin Mouse IgG 0
1 ABIN6055787 IF/ICC IHC WB Biotin Mouse IgG 0

General

抗原 Complement Factor H (CFH) 抗体
适用 小鼠, 大鼠, 人
(231), (34), (33), (3), (3), (3), (3), (2), (2)
宿主
(121), (85), (32), (16), (1), (1)
克隆类型
多克隆
标记 This Complement Factor H antibody is un-conjugated
(19), (12), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (1)
应用范围 Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(166), (118), (75), (42), (33), (26), (23), (22), (15), (10), (10), (9), (9), (5), (4), (1), (1), (1), (1)
Supplier

Complement Factor H抗体详情

Complement Factor H目标详情 使用细节 贮存及处理 图像
纯化方法 Affinity purification
免疫原 Recombinant protein of human CFH
亚型 IgG
Plasmids, Primers & others

Complement Factor H目标详情

Complement Factor H抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 CFH (CFH Antibody 摘要)
背景 CFH (Complement factor H) is a member of the Regulator of Complement Activation (RCA) gene cluster and a protein with twenty short concensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this CFH have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. 1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1, 33:D54-8.
Synonyms: ARMD4, ARMS1, CFHL3, FH, FHL1, HF, HF1, HF2, HUS, MGC88246
基因ID 3075
UniProt P08603
途径 Complement System, Cellular Response to Molecule of Bacterial Origin

使用细节

Complement Factor H抗体详情 Complement Factor H目标详情 贮存及处理 图像 回页首
应用备注 Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200
限制 仅限研究用

贮存及处理

Complement Factor H抗体详情 Complement Factor H目标详情 使用细节 图像 回页首
状态 Liquid
缓冲液 PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid freeze / thaw cycles
储存条件 -20 °C/-80 °C

图像

Complement Factor H抗体详情 Complement Factor H目标详情 使用细节 贮存及处理 回页首
厂商提供的图像
Western Blotting (WB) image for anti-Complement Factor H (CFH) antibody (ABIN3023097) Western blot analysis of extracts of HepG2 cell line, using CFH antibody.
'独立验证'标志
抗原 Commplement Factor H
Lot Number 4390101
Method validated Immunofluorescence
Positive Control MRL/lpr lupus mice (14 weeks of age)
Negative Control FH knockout MRL/lpr lupus mice
Notes Passed. The complement factor H antibody ABIN3023097 works very well on murine kidney samples and shows the expected CFH localization and strong staining.
Primary Antibody ABIN3023097
Secondary Antibody goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251)
实验流程
  • Prepare kidneys of wt and complement factor H ko MRL/lpr lupus mice of 14 weeks of age.
  • Snap-freeze kidneys in 2-methylbutane cooled on dry ice and keep at −80°C until use.
  • Sections were cut at 8µm using the cryostat.
  • Dry sections for 30min at RT.
  • Hydrate sections in PBS 2x for 15min at RT.
  • Fix cryostat sections with 4% paraformaldehyde (PFA) for 15min at RT.
  • Wash sections 2x with PBS for 15min at RT.
  • Incubate sections with primary rabbit anti-Complement Factor H antibody (antibodies-online, ABIN3023097, 0004390101) diluted 1:100 in PBS ON at 4°C.
  • Wash sections 2x for 15min with PBS.
  • Incubate sections with secondary goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251) diluted 1:100 in PBS for 30min at RT.
  • Wash sections 2x for 15min with PBS.
  • Incubate sections with ActinGreen 488 ReadyProbes Reagent (ThermoFisher Scientific, R37122, lot 1700569) diluted 1:100 in PBS for 30min at RT.
  • Wash sections 2x for 15min with PBS.
  • Image acquisition on a Zeiss microscope (Carl Zeiss, Oberkochen, Germany).
Validation Images
Immunofluorescence validation image for anti-Complement Factor H (CFH) antibody (ABIN3023097) IF staining with ABIN3023097 of kidneys harvested from 14 week old MRL/lpr mice suffi...