GFP-Booster (Atto 488)

ABIN509419 产品详细信息, 供应商: Log in to see
  • green fluorescent protein
  • gfp
Aequorea victoria
Recombinant Antibody
Atto 488
Fluorescence Microscopy (FM), Immunofluorescence (IF)
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原理 With our Booster you reactivate, boost, stabilizate the signals of your fusion proteins.
特异性 GFP-Booster efficiently detects and labels most common GFP derivates. No binding to red fluorescent proteins derived from DsRed can be detected.
  • Enhance, stabilize and reactivate your fl uorescent proteins
  • GFP-Booster highly specifi c for GFP fusion proteins (and derivatives thereof e.g. YFP or Venus)
  • Coupled to bright and photostable chemical dyes from ATTO-TEC
组件 GFP-Trap® coupled to fluorescent dye ATTO 488
别名 GFP
背景 Green fluorescent proteins (GFP) and variants thereof are widely used to study protein localization and dynamics in living cells. However, photo stability and quantum efficiency of GFP are not sufficient for Super-Resolution Microscopy (e.g. 3D-SIM or STED) of fixed samples. In addition, many cell biological methods such as BrdU-staining, EdU-Click-iT™ treatment or Fluorescent In Situ Hybridization result in disruption of the GFP signal.The GFP-Booster_Atto488, a specific GFP-binding protein coupled to the fluorescent dye ATTO 488, reactivates, boosts and stabilizes your GFP signal.
研究领域 Tags/Labels
应用备注 For the immunofluorescence staining of GFP-fusion proteins in fixed cells

ATTO 488:
Excitation range 480 - 510 nm (λabs= 501 nm)
Emission range 520 - 560 nm (λfl= 523 nm)

Booster are very small, highly specific GFP- or RFP-binding proteins covalently coupled to the superior fluorescent dyes from ATTO-TEC.

  • 1. Fixation: 4% paraformaldehyde (PFA) or 1:10 formalin (37% formaldehyde, 10-15% MetOH) in PBS, 10 min., RT.
  • 2. Wash 3x with PBS containing 0.1% Tween 20 (PBST). Critical: do not let coverslips “dry”.
  • 3. Permeabilisation: PBS containing 0.5% Triton X-100, 5 min., RT. Alternatively permeabilise by incubating in 100% methanol for 5min at -20°C.
  • 4. Wash 2x with PBST.
  • 5. Blocking: 4% BSA in PBST, 10 min, RT.
  • 6. GFP-Booster incubation: dilute GFP-Booster 1:200 in blocking buffer and incubate 1 h, RT. Note: For multiplexing protocols you can combine GFP-Booster with any other antibody.
  • 7. Wash 3x 5-10 min in PBST.
  • 8. If required counterstain with DNA fluorescent dyes, e.g. DAPI.
  • 9. Before mounting coverslips can be very briefly rinsed in water to prevent salt crystals to form.
  • 10. Mount in VectaShield (Vector Labs) or other mounting media with anti-fading agents and seal mounted coverslips with clear nail polish. Please note: Optimal dilutions/ concentrations should be determined by the end user
限制 仅限研究用
状态 Liquid
浓度 1 mg/ml
缓冲液 PBS, 0.01% Sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Do not freeze. Protect from light.
储存条件 4 °C
有效期 6 months
 image for GFP-Booster (Atto 488) (ABIN509419) Enhancement of GFP signal with GFP-Booster after EdU-Click-iT™ treatment. EdU-Click-i...
Immunofluorescence (IF) image for GFP-Booster (Atto 488) (ABIN509419) Visualization of GFP signal with GFP-Booster after EdU-Click-iT™ treatment. EdU-Click...
 image for GFP-Booster (Atto 488) (ABIN509419) Enhancement of GFP signal with GFP-Booster_Atto488. Comparison of signal intensity of...
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Vleugel, Tromer, Omerzu, Groenewold, Nijenhuis, Snel, Kops: "Arrayed BUB recruitment modules in the kinetochore scaffold KNL1 promote accurate chromosome segregation." in: The Journal of cell biology, Vol. 203, Issue 6, pp. 943-55, 2014 (PubMed).

Winterhoff, Junemann, Nordholz, Linkner, Schleicher, Faix: "The Diaphanous-related formin dDia1 is required for highly directional phototaxis and formation of properly sized fruiting bodies in Dictyostelium." in: European journal of cell biology, Vol. 93, Issue 5-6, pp. 212-24, 2014 (PubMed).

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Bleck, Itano, Johnson, Thomas, North, Bieniasz, Simon: "Temporal and spatial organization of ESCRT protein recruitment during HIV-1 budding." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 33, pp. 12211-6, 2014 (PubMed).

Bluteau, Zhuang, Amann, Trueb: "Targeted disruption of the intracellular domain of receptor FgfrL1 in mice." in: PLoS ONE, Vol. 9, Issue 8, pp. e105210, 2014 (PubMed).

Bourge, Fort, Soler, Satiat-Jeunemaître, Brown: "A pulse-chase strategy combining click-EdU and photoconvertible fluorescent reporter: tracking Golgi protein dynamics during the cell cycle." in: The New phytologist, 2014 (PubMed).

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Oliveira, Kotadia, Tavares, Mirkovic, Bowlin, Eichinger, Nasmyth, Sullivan: "Centromere-Independent Accumulation of Cohesin at Ectopic Heterochromatin Sites Induces Chromosome Stretching during Anaphase." in: PLoS biology, Vol. 12, Issue 10, pp. e1001962, 2014 (PubMed).