Antigen Retrieval Buffer pH 9.0

ABIN1689391 产品详细信息, 供应商: Log in to see
Immunohistochemistry (IHC)
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Supplier Product No.
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产品特性 This product is a solution that enables target retrieval in formalin-fixed, paraffin- embedded tissue section. It is optimal for use of primary antibodies that require Tris- EDTA buffer, pH 9.0 pretreatment.
实验流程 1. Dilute buffer 1:100 with deionized water before use. Example: 2 ml of concentrate diluted in 198 ml of deionized water.
2. Place slides in a coplin jar containing diluted Antigen Retrieval Buffer
3. Cover with a vented plastic wrap and place the jar in the microwave. Set at high power to boiling, then set at low power to keep it warm for 10 minutes.
4. Let the slides cool in the microwave for at least 20 minutes.
5. Wash slides with tap water for 1 minute.
6. Wash slides in rinse buffer twice for 3 minutes.
7. Proceed with appropriate staining protocol for the primary antibody in use.
限制 仅限研究用
状态 Liquid
浓度 100 X
缓冲液 Tris-EDTA Buffer, pH 9.0, with detergent. 100x concentrate.
注意事项 1. Take reasonable precautions when handling reagents. Wear appropriate Personal Protective Equipment.
2. Avoid contact of reagents with eyes, mucous membranes and skin. If reagents come into contact with sensitive areas, wash with copious amounts of water.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
储存条件 RT
储存方法 The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. Diluted reagent should be used promptly and is appropriate for one time use only.