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|抗原||Tumor Necrosis Factor (TNF) 抗体|
|适用||人, 小鼠, 灵长类, 大鼠 其他选择|
|标记||This Tumor Necrosis Factor antibody is un-conjugated 其他选择|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
|Supplier||Log in to see|
Tumor Necrosis Factor抗体详情Tumor Necrosis Factor目标详情 使用细节 贮存及处理 References for anti-Tumor Necrosis Factor antibody (ABIN153474) 图像
|特异性||The antiserum is directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNG beta (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.|
|纯化方法||Delipidation and Defibrination|
|免疫原||Recombinant full length protein produced in E. coli (Human).|
Tumor Necrosis Factor目标详情Tumor Necrosis Factor抗体详情 使用细节 贮存及处理 References for anti-Tumor Necrosis Factor antibody (ABIN153474) 图像 回页首
|别名||TNF-alpha (TNF Antibody 摘要)|
|背景||Gene Symbol: TNF|
|途径||NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus|
使用细节Tumor Necrosis Factor抗体详情 Tumor Necrosis Factor目标详情 贮存及处理 References for anti-Tumor Necrosis Factor antibody (ABIN153474) 图像 回页首
|应用备注||Western Blot 1:500 - 1:2000, ELISA 1:1000 - 1:5000, Immunohistochemistry 1:100 - 1:500, Immunohistochemistry-Paraffin 1:100 - 1:500|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
贮存及处理Tumor Necrosis Factor抗体详情 Tumor Necrosis Factor目标详情 使用细节 References for anti-Tumor Necrosis Factor antibody (ABIN153474) 图像 回页首
Buffer contains: No Preservative
|注意事项||Avoid freeze-thaw cycles|
|储存条件||4 °C,-20 °C|
|储存方法||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
References for anti-Tumor Necrosis Factor antibody (ABIN153474)Tumor Necrosis Factor抗体详情 Tumor Necrosis Factor目标详情 使用细节 贮存及处理 图像 回页首
Al-Khalaf, Amir, Al-Mohanna, Tulbah, Al-Sayed, Aboussekhra: "Obesity and p16(INK4A) Downregulation Activate Breast Adipocytes and Promote Their Protumorigenicity." in: Molecular and cellular biology, Vol. 37, Issue 17, 2017 (Sample species: Human).
Rossi, Cadamuro, Biselli-Périco, Leite, Severino, Reis, Cordeiro, Silva: "Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection." in: Cellular microbiology, Vol. 18, Issue 10, pp. 1444-58, 2016 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Chen, Jian, Lien, Lin, Ting, Chen, Hsu, Huang, Wu, Kuan, Chao, Wu, Huang, Juan: "Adipocytes play an etiological role in the podocytopathy of high-fat diet-fed rats." in: The Journal of endocrinology, 2016 (Sample species: Rat (Rattus)). Further details: Western Blotting
Günel, Demirci, Eryılmaz, Yılmaz, Meteoğlu, Ömürlü, Başal: "Inhibitory Effect of Pycnogenol(®) on Airway Inflammation in Ovalbumin-Induced Allergic Rhinitis." in: Balkan medical journal, Vol. 33, Issue 6, pp. 620-626, 2016 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Rat (Rattus)).
Chen, Zhang, Wang, Lei, Su, Zeng, Pei, Huang: "Inhibition of immunoproteasome reduces infarction volume and attenuates inflammatory reaction in a rat model of ischemic stroke." in: Cell death & disease, Vol. 6, pp. e1626, 2015 (Sample species: Rat (Rattus)). Further details: Western Blotting
Tessier, Raha, Holloway, Yockell-Lelièvre, Tayade, Gruslin: "Characterization of immune cells and cytokine localization in the rat utero-placental unit mid- to late gestation." in: Journal of reproductive immunology, Vol. 110, pp. 89-101, 2015 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Wang, Parrish: "Loss of α(E)-catenin promotes Fas mediated apoptosis in tubular epithelial cells." in: Apoptosis : an international journal on programmed cell death, Vol. 20, Issue 7, pp. 921-9, 2015 (Sample species: Rat (Rattus)). Further details: Western Blotting
Rodriguez, Edrey, Osmulski, Gaczynska, Buffenstein: "Altered composition of liver proteasome assemblies contributes to enhanced proteasome activity in the exceptionally long-lived naked mole-rat." in: PLoS ONE, Vol. 7, Issue 5, pp. e35890, 2012 Further details: Western Blotting
Roth, Städler, Lemann, Hösli, Simon, Simon: "Distinct eosinophil cytokine expression patterns in skin diseases - the possible existence of functionally different eosinophil subpopulations." in: Allergy, Vol. 66, Issue 11, pp. 1477-86, 2011 (Sample species: Human). Further details: Immunohistochemistry
图像Tumor Necrosis Factor抗体详情 Tumor Necrosis Factor目标详情 使用细节 贮存及处理 References for anti-Tumor Necrosis Factor antibody (ABIN153474) 回页首