小鼠 anti-人 IgG (Heavy & Light Chain) Antibody

ABIN969481 产品详细信息, 供应商: Log in to see
抗原
抗原表位
Heavy & Light Chain
4679
2536
1881
1066
1006
860
300
294
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
适用

2847
2739
2297
1675
1432
788
613
442
380
379
358
339
291
290
208
138
135
122
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
宿主
小鼠
6708
4830
1795
890
609
388
91
55
52
21
8
5
5
5
2
1
1
克隆类型 (克隆位点)
单克隆 ()
应用范围
ELISA, Western Blotting (WB)
选项
Supplier
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免疫原 Human IgG was isolated from human sera and purified by chromatography.
克隆位点 4D2D9G8-4A10
亚型 IgG1
产品特性 Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
纯化方法 Purified
背景 Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
分子量 50 kDa
研究领域 Immunology, Secondary Antibodies
应用备注 Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
限制 仅限研究用
状态 Liquid
缓冲液 PBS containing 0.03 % sodium azide.
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
储存条件 4 °C/-20 °C
储存方法 Store at 4 °C or at -20 °C for long term.
厂商提供的图像
Western Blotting (WB) image for Mouse anti-Human IgG (Heavy & Light Chain) antibody (ABIN969481) Figure 1: Western blot analysis using IgG mouse mAb (lane 1, 2) and Albumin mouse mAb...
有引用在: Klonisch, Panayotou, Edwards, Jackson, Berger, Delves, Lund, Roitt: "Enhancement in antigen binding by a combination of synergy and antibody capture." in: Immunology, Vol. 89, Issue 2, pp. 165-71, 1996 (PubMed).