PRKAR2B 抗体 (pSer114)
(2 references)-
- 抗原 See all PRKAR2B 抗体
- PRKAR2B (Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B))
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抗原表位
- pSer114
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适用
- 人, 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This PRKAR2B antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Fluorescence Microscopy (FM)
- 交叉反应
- 小鼠, 大鼠
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Phosphorylated Human PKA[RIIbeta] peptide Peptide
- 克隆位点
- 47-PKA
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product PRKAR2B Primary Antibody
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anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (Ser113) antibody
PRKAR2B 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (Internal Region) antibodyPRKAR2B 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (Ser113) antibodyPRKAR2B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (AA 32-62), (N-Term) antibodyPRKAR2B 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB3253 unconjugated
anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (pSer113) antibodyPRKAR2B 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (pSer113) antibodyPRKAR2B 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (AA 304-413) antibodyPRKAR2B 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (AA 1-418) antibodyPRKAR2B 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (AA 32-62) antibodyPRKAR2B 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (pSer113) antibodyPRKAR2B 适用: 人, 小鼠, 大鼠, 猴, 兔 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS. - 说明
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Related Products: ABIN968545
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20° C.
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: "Point mutation of the autophosphorylation site or in the nuclear location signal causes protein kinase A RII beta regulatory subunit to lose its ability to revert transformed fibroblasts." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 23, pp. 10634-8, (1995) (PubMed).
: "Phosphorylation of the regulatory subunit of type II beta cAMP-dependent protein kinase by cyclin B/p34cdc2 kinase impairs its binding to microtubule-associated protein 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 12, pp. 5418-22, (1993) (PubMed).
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: "Point mutation of the autophosphorylation site or in the nuclear location signal causes protein kinase A RII beta regulatory subunit to lose its ability to revert transformed fibroblasts." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 23, pp. 10634-8, (1995) (PubMed).
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- 抗原
- PRKAR2B (Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B))
- 别名
- PKA RIIbeta (PRKAR2B 产品)
- 别名
- MGC83177 antibody, PRKAR2B antibody, AI451071 antibody, AW061005 antibody, PKARIIbeta antibody, Pkarb2 antibody, RII(beta) antibody, RATDNA antibody, PRKAR2 antibody, RII-BETA antibody, protein kinase, cAMP-dependent, regulatory subunit type II beta S homeolog antibody, protein kinase cAMP-dependent type II regulatory subunit beta antibody, protein kinase, cAMP-dependent, regulatory, type II, beta antibody, protein kinase, cAMP dependent regulatory, type II beta antibody, protein kinase cAMP-dependent type 2 regulatory subunit beta antibody, prkar2b.S antibody, PRKAR2B antibody, prkar2b antibody, Prkar2b antibody
- 背景
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CAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory (R). Four regulatory subunits have been identified: RIalpha, RIbeta, RIIalpha, and RIIbeta. These subunits define type I and II PKAs. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and type II holoenzymes have three potential C subunits (Calpha, Cbeta, or Cgamma). Most cells, including T lymphocytes, express both type I and type II PKAs. RIIa expression is associated with cellular transformation, while RIIbeta expression correlates with mitotic arrest and cellular differentiation. Type II PKA can be distinguished by autophosphorylation of the R subunits, while type I PKA binds Mg/ATP with high affinity. The cAMP-dependent autophosphorylation of the human RIIbeta subunits occurs at serine 114 (S114). In addition to their enzyme regulatory activity, the RIIalpha and RIIbeta subunits determine the subcellular location of the holoenzymes via their interactions with specific intracellular anchoring proteins.
The 47/PKA monoclonal antibody recognizes the phosphorylated S114 in the RIIbeta subunit of PKA. The orthologous phosphorylation site in mouse and rat PKA[RIIbeta] is S112. - 分子量
- 53 kDa
- 途径
- Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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