AP2B1 抗体 (AA 75-245)
(5 references)-
- 抗原 See all AP2B1 抗体
- AP2B1 (Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1))
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抗原表位
- AA 75-245
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适用
- 人, 小鼠, 大鼠, 犬, 小鸡, Frog
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This AP2B1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), BioImaging (BI)
- 交叉反应
- 小鼠, 大鼠, 犬, 小鸡, Frog
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Please refer to us for technical protocols. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human Adaptin beta aa. 75-245
- 克隆位点
- 74-Adaptin beta
- 亚型
- IgG1
- Top Product
- Discover our top product AP2B1 Primary Antibody
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anti-Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1) (AA 752-951) antibody
AP2B1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1) antibodyAP2B1 适用: 人, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1) (AA 738-937) antibodyAP2B1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1) (AA 585-651) antibodyAP2B1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2D5 unconjugated
anti-Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1) (AA 585-652) antibodyAP2B1 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2D5 unconjugated
anti-Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1) (Internal Region) antibodyAP2B1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1) (C-Term) antibodyAP2B1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 豚鼠, 马, 斑马鱼, 小鸡, 非洲爪蟾, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1) (AA 497-546) antibodyAP2B1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 豚鼠, 马, 斑马鱼, 小鸡, 非洲爪蟾, 猴, Pig, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1) (AA 651-700) antibodyAP2B1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 豚鼠, 马, 小鸡, 非洲爪蟾, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1) (Middle Region) antibodyAP2B1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 豚鼠, 马, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument. - 说明
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Related Products: ABIN968537, ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20°C.
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: "Phosphoinositide 3-kinase regulates beta2-adrenergic receptor endocytosis by AP-2 recruitment to the receptor/beta-arrestin complex." in: The Journal of cell biology, Vol. 158, Issue 3, pp. 563-75, (2002) (PubMed).
: "Lipid rafts are required for GLUT4 internalization in adipose cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 21, pp. 12050-5, (2001) (PubMed).
: "The beta2-adrenergic receptor/betaarrestin complex recruits the clathrin adaptor AP-2 during endocytosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 7, pp. 3712-7, (1999) (PubMed).
: "Conservation and diversity in families of coated vesicle adaptins." in: The Journal of biological chemistry, Vol. 265, Issue 9, pp. 4814-20, (1990) (PubMed).
: "Structural and functional division into two domains of the large (100- to 115-kDa) chains of the clathrin-associated protein complex AP-2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 8, pp. 2612-6, (1989) (PubMed).
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: "Phosphoinositide 3-kinase regulates beta2-adrenergic receptor endocytosis by AP-2 recruitment to the receptor/beta-arrestin complex." in: The Journal of cell biology, Vol. 158, Issue 3, pp. 563-75, (2002) (PubMed).
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- 抗原
- AP2B1 (Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1))
- 别名
- Adaptin beta (AP2B1 产品)
- 别名
- 5-HT(2B) antibody, 5-HT2B antibody, ADTB2 antibody, AP105B antibody, AP2-BETA antibody, CLAPB1 antibody, 1300012O03Rik antibody, AI788979 antibody, fa16e07 antibody, wu:fa16c06 antibody, wu:fa16e07 antibody, wu:fc18c08 antibody, zgc:55659 antibody, 5-hydroxytryptamine receptor 2B antibody, adaptor related protein complex 2 beta 1 subunit antibody, adaptor-related protein complex 2, beta 1 subunit antibody, adaptor related protein complex 2 beta 1 subunit L homeolog antibody, HTR2B antibody, AP2B1 antibody, Ap2b1 antibody, ap2b1 antibody, ap2b1.L antibody
- 背景
- Sorting of integral membrane proteins at various stages of the endocytic and secretory pathways is mediated by vesicular trafficking between a variety of organelles. Two sorting signals are tyrosine-based and dileucine-based signals that interact with heterotetrameric adaptor protein complexes (AP-1, AP-2, AP-3, and AP-4), which are associated with the vesicle coats. These coatomers contain two large Adaptin proteins (gamma, alpha, delta, or epsilon and beta1, beta2, beta3, or beta4, respectively) that are noncovalently linked to one medium chain (µ1, µ2, µ3, or µ4) and one small chain (sigma1, sigma2, sigma3, or sigma4). The AP-1 and AP-3 complexes are involved in protein sorting from the TGN and endosomes, while AP-2 adaptor complexes are involved in clathrin-mediated endocytosis. beta Adaptin subunits (beta1, beta2, beta3, beta4) lack sequence homology to adaptins alpha, gamma, delta, and epsilon, but all of these subunits share a similar domain structure. Adaptin beta1 (also known as Adaptin beta') and beta2 (also known as Adaptin beta) have 83% amino acid identity and are found in the AP1 and AP2 complexes, respectively.
- 分子量
- 106 kDa
- 途径
- EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation
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