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His Tag 抗体

WB, IP 宿主: 小鼠 Monoclonal F24-796 unconjugated
产品编号 ABIN967362
发货至: 中国
  • 抗原 See all His Tag 抗体
    His Tag
    适用
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    宿主
    • 93
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    小鼠
    克隆类型
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    • 10
    单克隆
    标记
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    This His Tag antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunoprecipitation (IP)
    品牌
    BD Pharmingen™
    序列
    HHHHHH
    产品特性
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    纯化方法
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    克隆位点
    F24-796
    亚型
    IgG2b
    Top Product
    Discover our top product His Tag Primary Antibody
  • 应用备注
    An E. coli produced recombinant 6xHis control lysate is provided as a positive control. Store lysates at -20°C.
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store undiluted at 4°C.
  • Hochuli, Döbeli, Schacher: "New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues." in: Journal of chromatography, Vol. 411, pp. 177-84, (1988) (PubMed).

  • 抗原
    His Tag
    别名
    6xHis (His Tag 产品)
    物质类
    Tag
    背景
    The engineering of a short epitope tag to a recombinant protein provides a very convenient method for detecting and isolating the protein of interest. For example, the placement of a series of 6 consecutive histidine residues that are incorporated at either end of the recombinant fusion protein facilitates the purification of that protein. Purification can be easily accomplished by metal chelate affinity chromatography using a metal resin column. The 6xHis monoclonal antibody can be used to detect the epitope-tagged protein of interest.
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