p34 Cdc-2protein (Nuclear) 抗体
适用: 人
IHC, WB
宿主: 小鼠
Monoclonal
B322 (POH-1)
unconjugated
-
- 抗原
- p34 Cdc-2protein
- 抗原表位
- Nuclear
- 适用
- 人
- 宿主
- 小鼠
- 克隆类型
- 单克隆
- 应用范围
- Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- This antibody reacts with a 34 kD cyclic dependent serine/threonine kinase protein. P34 cdc2 and cyclin B1 are catalytic subunits of the maturation promoting factor (MPF). This antigen is activated with cyclins through dephosphrylation of tyrosine residue, initiates the entry of cells into mitosis, and is followed by the breakdown of the nuclear envelope. Expression of this antigen reflects the proliferating potential of positively staining tissue.
- 纯化方法
- Concentrated.
- 免疫原
- The recombinant human p34.cdc2 fusion protein.
- 克隆位点
- B322 (POH-1)
- 亚型
- IgG2a
-
- 应用备注
- Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.5 mg/ml.
- 缓冲液
- 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
-
- 抗原
- p34 Cdc-2protein
- 基因ID
- 983
-
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