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p105 Proliferating-Associated Nuclear Antigen (Nuclear) 抗体

适用: 人 IHC (fp), WB 宿主: 小鼠 Monoclonal B301 unconjugated
产品编号 ABIN966725
发货至: 中国
  • 抗原
    p105 Proliferating-Associated Nuclear Antigen
    抗原表位
    Nuclear
    适用
    宿主
    小鼠
    克隆类型
    单克隆
    应用范围
    Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Western Blotting (WB)
    特异性
    The p105 protein, found in all human cells in a dimeric form (105 kD) and monomeric form (41 kD), is a proliferation-associated nuclear antigen that is absent in non-cycling cells in the G0 phase. This protein is important in the synthesis and transport of RNA in the cell regulation cycle. During G2 and mitosis, this protein is increased possibly due to the unmasking of nucleolar antigens, resulting in large accumulations of this protein in the mitotic cytoplasm.
    纯化方法
    Concentrated.
    免疫原
    Activated peripheral blood nuclei.
    克隆位点
    B301
    亚型
    IgM
  • 应用备注
    Immunohistochemistry: 1:50-1:100. Formalin-fixed paraffin-embedded (FFPE) tissue sections require pepsin or trypsin digestion.
    Western blotting: 1:100-1:200 .
    Staining Procedure: This antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. FFPE tissue sections require pepsin or trypsin digestion. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.
    说明

    Cellular Localization: Nuclear.
    Recommended Positive Control: Human tonsil or lymph node.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.25 mg/ml.
    缓冲液
    20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
  • 抗原
    p105 Proliferating-Associated Nuclear Antigen
    分子量
    Dimeric form 105 kDa, Monomeric form 41 kDa
    基因ID
    4790
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