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山羊 anti-小鼠 IgG (Fc Region) Antibody - Preadsorbed

ELISA, IHC, WB Polyclonal IgG unconjugated F(ab')2 fragment
产品编号 ABIN965159
发货至: 中国
  • 抗原 See all IgG products
    IgG
    片段
    F(ab')2 fragment
    抗原表位
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    Fc Region
    适用
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    小鼠
    宿主
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    山羊
    克隆类型
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    多克隆
    应用范围
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum.
    纯化方法
    Preadsorption: Solid phase absorption
    过滤
    Sterile filtered
    免疫原

    Immunogen: Mouse IgG F(c) fragment

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

    ELISA Dilution: 1:10,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.9 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4 °C prior to opening. This product is stable for several weeks at 4 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -24 °C or below.
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: Goat F(ab')2 Anti-Mouse IgG F(c) Antibody, Goat Fab2 Anti-Mouse IgG Fc Antibody

    Background: F(ab')2 Anti-Mouse IgG F(c) Antibody was generated in goat and detects specifically Mouse IgG F(c). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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