兔 anti-Golden Syrian Hamster IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed
(1 reference)-
- 抗原 See all IgG products
- IgG
- 片段
- F(ab')2 fragment
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抗原表位
- Heavy & Light Chain
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适用
- Golden Syrian Hamster
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- Alkaline Phosphatase (AP)
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Golden Syrian Hamster IgG whole molecule
- 亚型
- IgG
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Goat anti-Armenian Hamster IgG (Heavy & Light Chain) antibody - Preadsorbed
ELISA, IHC, WB IgG unconjugated
Rabbit anti-Golden Syrian Hamster IgG (Heavy & Light Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated F(ab')2 fragment
Goat anti-Golden Syrian Hamster IgG (Heavy & Light Chain) antibody (HRP)ELISA, WB Polyclonal IgG HRP
Goat anti-Golden Syrian Hamster IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Armenian Hamster IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin F(ab')2 fragment
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- 应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Hamster IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:3,000 - 1:12,000
Western Blot Dilution: 1:500 - 1:2,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.6 mg/mL
- 缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - 储存条件
- 4 °C
- 储存方法
- Store vial at 4 °C
- 有效期
- 12 months
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: "The glutamate agonist NMDA blocks gonadal regression and enhances antibody response to an immune challenge in Siberian hamsters (Phodopus sungorus)." in: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, Vol. 180, Issue 2, pp. 267-77, (2010) (PubMed).
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: "The glutamate agonist NMDA blocks gonadal regression and enhances antibody response to an immune challenge in Siberian hamsters (Phodopus sungorus)." in: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, Vol. 180, Issue 2, pp. 267-77, (2010) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: Rabbit F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG Antibody Alkaline Phosphatase conjugation, Rabbit F(ab')2 Anti-Hamster IgG Alkaline Phosphatase Conjugated Antibody, Rabbit Fab2 Anti-Hamster IgG alk phos Conjugated Antibody
Background: F(ab')2 Anti-Golden Syrian Hamster IgG Alkaline Phosphatase Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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