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兔 anti-Cow IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG FITC F(ab')2 fragment
产品编号 ABIN965016
发货至: 中国
  • 抗原 See all IgG products
    IgG
    片段
    F(ab')2 fragment
    抗原表位
    • 2332
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    Heavy & Light Chain
    适用
    • 692
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    Cow
    宿主
    • 1718
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    克隆类型
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    • 1
    多克隆
    标记
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    FITC
    应用范围
    • 1837
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    • 234
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    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Bovine IgG and Bovine Serum.
    产品特性
    This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    纯化方法
    Preadsorption: Solid phase absorption
    过滤
    Sterile filtered
    免疫原

    Immunogen: Bovine IgG whole molecule

    亚型
    IgG
    Labeling Ratio
    3.64
  • 应用备注

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    说明

    Excitation/Emission wavelength: 494 nm/514 nm

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 500 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Product is photosensitive and should be protected from light.
    Avoid cycles of freezing and thawing.
    Do NOT add Sodium Azide!
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: Rabbit F(ab')2 Anti-Bovine fluorescein Conjugated Antibody, Rabbit Fab2 Anti-Bovine fluorescein Conjugated Antibody, Rabbit Fab'2 Anti-Bovine Antibody FITC Conjugation

    Background: F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

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