山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody (Atto 647N) - Preadsorbed
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Atto 647N
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应用范围
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum.
- 产品特性
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Anti-Rat IgG (H&L) conjugated to ATTO 647N is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. - 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Rat IgG whole molecule
- 亚型
- IgG
- Labeling Ratio
- 1.68
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- 应用备注
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Application Note: The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
FLISA Dilution: >1:20,000
Western Blot Dilution: >1:10,000
IF Microscopy Dilution: >1:5,000
- 说明
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The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light. - 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
- 有效期
- 12 months
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Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons." in: PLoS ONE, Vol. 13, Issue 8, pp. e0200759, (2019) (PubMed).
: "Neurogenetic dissection of the Drosophila lateral horn reveals major outputs, diverse behavioural functions, and interactions with the mushroom body." in: eLife, Vol. 8, (2019) (PubMed).
: "Angular velocity integration in a fly heading circuit." in: eLife, Vol. 6, (2018) (PubMed).
: "Neuroarchitecture of the Drosophila central complex: A catalog of nodulus and asymmetrical body neurons and a revision of the protocerebral bridge catalog." in: The Journal of comparative neurology, Vol. 526, Issue 16, pp. 2585-2611, (2018) (PubMed).
: "Feature Integration Drives Probabilistic Behavior in the Drosophila Escape Response." in: Neuron, Vol. 94, Issue 6, pp. 1190-1204.e6, (2017) (PubMed).
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Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons." in: PLoS ONE, Vol. 13, Issue 8, pp. e0200759, (2019) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: Goat anti-Rat IgG ATTO647N Conjugated Antibody, Goat anti-Rat IgG Antibody ATTO 647N Conjugation
Background: Anti-Rat IgG (H&L) conjugated to ATTO 647N is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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