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兔 anti-小鼠 IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Biotin
产品编号 ABIN964974
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2332
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
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    • 1
    Heavy & Light Chain
    适用
    • 691
    • 672
    • 559
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    小鼠
    宿主
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    克隆类型
    • 3346
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    • 1
    多克隆
    标记
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    Biotin
    应用范围
    • 1836
    • 1788
    • 1787
    • 1498
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    • 515
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    Mouse secondary antibodyAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
    交叉反应
    小鼠
    产品特性
    Mouse secondary antibody conjugated to biotin is available in a variety of formats. Anti-IgG Secondary Antibody conjugate is Suitable for western blot, ELISA and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency.
    The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Mouse IgG whole molecule

    亚型
    IgG
    Labeling Ratio
    10-20
  • 应用备注

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Mouse secondary antibody conjugated to biotin is available in a variety of formats. Anti-IgG Secondary Antibody conjugate is Suitable for western blot, ELISA and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency.

    ELISA Dilution: 1:300,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    说明

    When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    2.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid cycles of freezing and thawing.
    Dilute only prior to immediate use
    This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20 °C or below after dilution.
    储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vial at -20 °C or below prior to opening.
    有效期
    12 months
  • Judd, Bredin, Kalantzis, Jenkins, Ernst, Giraud: "STAT3 activation regulates growth, inflammation, and vascularization in a mouse model of gastric tumorigenesis." in: Gastroenterology, Vol. 131, Issue 4, pp. 1073-85, (2006) (PubMed).

    Howlett, Judd, Jenkins, La Gruta, Grail, Ernst, Giraud: "Differential regulation of gastric tumor growth by cytokines that signal exclusively through the coreceptor gp130." in: Gastroenterology, Vol. 129, Issue 3, pp. 1005-18, (2005) (PubMed).

  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: rabbit anti-Mouse IgG Antibody biotin conjugation, rabbit anti-Mouse IgG biotin conjugated Antibody

    Background: Anti-Mouse IgG Biotin Antibody generated in rabbit detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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