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山羊 anti-小鼠 IgG (Heavy & Light Chain) Antibody (Atto 594) - Preadsorbed

WB, FLISA, FM Polyclonal IgG Atto 594
产品编号 ABIN964962
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2332
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
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    • 15
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    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    适用
    • 691
    • 672
    • 559
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    • 366
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    • 3
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    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    小鼠
    宿主
    • 1717
    • 1474
    • 537
    • 203
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    • 103
    • 33
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    • 1
    山羊
    克隆类型
    • 3346
    • 164
    • 1
    多克隆
    标记
    • 556
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    Atto 594
    应用范围
    • 1837
    • 1789
    • 1787
    • 1498
    • 962
    • 933
    • 912
    • 515
    • 234
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    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
    特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.
    产品特性
    Anti-Mouse IgG (H&L) conjugated to ATTO 594 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Mouse IgG whole molecule

    亚型
    IgG
    Labeling Ratio
    3.0
  • 应用备注

    Application Note: Anti-Mouse IgG (H&L) conjugated to ATTO 594 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

    FLISA Dilution: >1:20,000

    Western Blot Dilution: >1:10,000

    IF Microscopy Dilution: >1:5,000

    说明

    The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 500 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid cycles of freezing and thawing.
    This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Product is photosensitive and should be protected from light.
    储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vial at -20 °C or below prior to opening. Store the vial at -20 °C or below after dilution.
    有效期
    12 months
  • Purkey, Woolfrey, Crosby, Stich, Chick, Aoto, DellAcqua: "AKAP150 Palmitoylation Regulates Synaptic Incorporation of Ca2+-Permeable AMPA Receptors to Control LTP." in: Cell reports, Vol. 25, Issue 4, pp. 974-987.e4, (2018) (PubMed).

  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: Goat Anti-Mouse IgG Antibody ATTO594 Conjugation, Goat Anti-Mouse IgG ATTO 594 Conjugated Antibody

    Background: Anti-Mouse IgG ATTO 594 Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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