山羊 anti-小鼠 IgG (Heavy & Light Chain) Antibody (Atto 488)

ABIN964957 产品详细信息, 供应商: Log in to see
抗原
抗原表位
Heavy & Light Chain
6223
2028
1776
1135
782
648
372
305
34
34
31
15
12
12
12
12
11
7
3
3
1
1
1
1
1
1
1
适用
小鼠
3074
2909
2377
1662
1508
784
737
489
476
410
401
395
341
297
271
160
158
111
54
34
30
26
16
15
12
10
8
7
5
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
宿主
山羊
7123
5281
1970
1077
507
359
89
84
51
35
23
15
8
5
1
1
1
克隆类型
多克隆
标记
Atto 488
2218
1820
1751
1266
793
717
430
343
235
147
145
140
140
116
112
112
102
98
94
93
92
91
89
84
81
79
78
75
73
72
68
68
65
61
55
54
54
54
54
53
53
53
53
53
53
52
48
43
41
37
32
31
23
23
22
20
19
18
17
16
15
15
15
14
14
14
12
12
12
12
12
11
11
10
10
10
10
9
9
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
应用范围
FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
选项
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免疫原

Immunogen: Mouse IgG whole molecule

亚型 IgG
特异性 Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.
交叉反应 (详细) Pre-Adsorbed
产品特性 Anti-Mouse IgG (H&L) conjugated by ATTO 488 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
纯化方法 Mouse IgG (H&L) Antibody ATTO 488 was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.  This antibody will react with heavy chains of mouse IgG and with light chains of most mouse immunoglobulins.
过滤 Sterile filtered
Labeling Ratio 3.0
抗原
背景

Synonyms: Goat Anti-Mouse IgG Antibody ATTO488 Conjugation, Goat Anti-Mouse IgG ATTO 488 Conjugated Antibody

Background: Anti-Mouse IgG ATTO 488 Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

应用备注

Application Note: Anti-Mouse IgG (H&L) conjugated by ATTO 488 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

说明

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

限制 仅限研究用
状态 Lyophilized
溶解方式

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

浓度 1.0 mg/mL
缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light.
储存条件 RT,4 °C,-20 °C
储存方法 Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
有效期 12 months
厂商提供的图像
Western Blotting (WB) image for Goat anti-Mouse (Murine) IgG (Heavy & Light Chain) antibody (Atto 488) (ABIN964957) Western Blot of ATTO 488 conjugated Goat anti-Mouse IgG Pre-adsorbed secondary antibo...
 image for Goat anti-Mouse (Murine) IgG (Heavy & Light Chain) antibody (Atto 488) (ABIN964957) Goat anti-Mouse (Murine) IgG (Heavy & Light Chain) antibody (Atto 488) (Image 2)