AKT2 抗体 (AA 455-468)
(1 reference)-
- 抗原 See all AKT2 抗体
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
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抗原表位
- AA 455-468
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This AKT2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 免疫原
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 455-468 (of 481) of human AKT2 conjugated to Keyhole Limpet Hemocyanin (KLH).
- 亚型
- IgG
- Top Product
- Discover our top product AKT2 Primary Antibody
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anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 100-189) antibody
AKT2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1D9 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 100-189) antibodyAKT2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal X1 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 100-189) antibodyAKT2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F8 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (Internal Region) antibodyAKT2 适用: 人 WB, ELISA, IHC 宿主: 大鼠 Monoclonal 16G11-A7 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 93-123) antibodyAKT2 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 416-444) antibodyAKT2 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 142-412) antibodyAKT2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (C-Term) antibodyAKT2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (N-Term) antibodyAKT2 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (pSer474) antibodyAKT2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- This antibody was tested by ELISA and immunoblotting and was found to be reactive with both unphosphorylated and phosphorylated AKT2 in a lysate of HEK293 cells. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.This product has been assayed by immunoblot against a HEK293 cell lysate and is reactive at a 1:1,000 dilution showing a band at approximately 60 kDa. A working dilution of 1:4,000 to 1:16,000 is suggested for this product in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature against 0.1 ug of the immunizing peptide. Researchers should determine optimal titers for other applications.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
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: "Inhibition of protein kinase Akt1 by apoptosis signal-regulating kinase-1 (ASK1) is involved in apoptotic inhibition of regulatory volume increase." in: The Journal of biological chemistry, Vol. 285, Issue 9, pp. 6109-17, (2010) (PubMed).
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: "Inhibition of protein kinase Akt1 by apoptosis signal-regulating kinase-1 (ASK1) is involved in apoptotic inhibition of regulatory volume increase." in: The Journal of biological chemistry, Vol. 285, Issue 9, pp. 6109-17, (2010) (PubMed).
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- 抗原
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
- 别名
- AKT2 (AKT2 产品)
- 别名
- AKT2 antibody, pkbb antibody, pkbbeta antibody, pkbss antibody, prkbb antibody, rac-beta antibody, v-akt2 antibody, 2410016A19Rik antibody, AW554154 antibody, PKB antibody, PKBbeta antibody, HIHGHH antibody, PKBB antibody, PKBBETA antibody, PRKBB antibody, RAC-BETA antibody, cb945 antibody, akt2-a antibody, AKT serine/threonine kinase 2 antibody, v-akt murine thymoma viral oncogene homolog 2 antibody, thymoma viral proto-oncogene 2 antibody, v-akt murine thymoma viral oncogene homolog 2 L homeolog antibody, AKT2 antibody, akt2 antibody, Akt2 antibody, akt2.L antibody
- 背景
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AKT2 is also known as V-AKT Murine Thymoma Viral Oncogene Homolog 2 antibody, Rac protein kinase beta antibody, PKB beta antibody or PRKBB antibody. AKT2 is an isoform of the phosphoinositide-dependent serine-threonine protein kinase AKT and is enriched in insulin-responsive tissues and has been implicated in the metabolic actions of the hormone. AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Over-expression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins. AKT2 mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT2 to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT2 dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT2 has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
Synonyms: AKT 2 antibody, AKT-2, PKB antibody, PKB beta antibody, PKBBETA antibody, PRKBB antibody, Protein kinase Akt 2 antibody, Protein kinase B beta antibody, RAC-beta serine/threonine-protein kinase, RAC-PK-beta - 基因ID
- 208, 164731
- UniProt
- P31751
- 途径
- PI3K-Akt Signaling, RTK signaling, AMPK Signaling, TLR signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
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