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CD48 抗体

Name: CD48 抗体
Brand: antibodies-online
SKU: ABIN94157
Availability: OutOfStock

CD48 适用: 人, Non-Human Primate FACS, WB, IP, IHC (p) 宿主: 小鼠 Monoclonal MEM-102 unconjugated

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD48 (MEM-102) purified antibody (concentration in sample 3 μg/mL, GAM APC).

FACS

Surface staining of human peripheral blood cells with anti-CD48 (MEM-102) Alexa Fluor® 700.

FACS

Separation of CD48 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD48 (MEM-102) purified antibody (concentration in sample 3 μg/mL, GAM APC).

FACS

3 images

(5 references)

产品编号 ABIN94157

发货至: 中国

Datasheet as PDF

抗原 See all CD48 抗体

CD48
适用
86

42

14

6

5

4

4

2

1

1

1

1
人, Non-Human Primate
宿主
55

35

14

6

2
小鼠
Compatible Secondaries
克隆类型
76

33
单克隆
标记
46

11

11

10

6

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
This CD48 antibody is un-conjugated
应用范围
72

40

19

15

14

12

10

6

5

3

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

特异性

The antibody MEM-102 reacts with CD48 (Blast-1), a 40-47 kDa GPI-anchored extracellular membrane protein (immunoglobulin supergene family) widely expressed on hematopoietic cells, it is negative on granulocytes, platelets and erythrocytes.

交叉反应（详细）

Human, Non-Human Primates

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

Raji human Burkitt's lymphoma cell line

克隆位点

MEM-102

亚型

IgG1

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CD48 适用: 人, Non-Human Primate FACS, WB, IP, IHC (p) 宿主: 小鼠 Monoclonal MEM-102 Biotin

anti-CD48 (CD48) (AA 27-220) antibody
CD48 适用: 人 FACS, WB, ELISA, IF 宿主: 小鼠 Monoclonal 6F9H2 unconjugated

anti-CD48 (CD48) (AA 27-220) antibody
CD48 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM43 unconjugated Recombinant Antibody

anti-CD48 (CD48) antibody (FITC)
CD48 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-102 FITC

anti-CD48 (CD48) antibody
CD48 适用: 人 WB 宿主: 兔 Monoclonal unconjugated

anti-CD48 (CD48) (AA 1-243) antibody
CD48 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-CD48 (CD48) (AA 1-169) antibody
CD48 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3B8-2C2 unconjugated

anti-CD48 (CD48) antibody (APC)
CD48 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-102 APC

anti-CD48 (CD48) antibody (PE)
CD48 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-102 PE

anti-CD48 (CD48) (AA 47-181) antibody
CD48 适用: 人 FACS, WB, ELISA, IHC, ICC, Neut 宿主: 小鼠 Monoclonal 2B11A3 unconjugated

应用备注

Flow cytometry: Recommended dilution: 2 μg/mL.
Immunohistochemistry: Recommended dilution: 5-10 μg/mL.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Schatzlmaier, Supper, Göschl, Zwirzitz, Eckerstorfer, Ellmeier, Huppa, Stockinger: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

Angelisová, Drbal, Horejsí, Cerný: "Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase." in: Blood, Vol. 93, Issue 4, pp. 1437-9, (1999) (PubMed).

Stulnig, Berger, Sigmund, Stockinger, Horejsí, Waldhäusl: "Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol." in: The Journal of biological chemistry, Vol. 272, Issue 31, pp. 19242-7, (1997) (PubMed).

Bazil, Stefanová, Hilgert, Kristofová, Van?k, Bukovský, Horejsí: "Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102." in: Folia biologica, Vol. 35, Issue 5, pp. 289-97, (1990) (PubMed).
抗原

CD48

别名

CD48 (CD48 产品)

别名

AI449234 antibody, AW610730 antibody, BCM1 antibody, BLAST antibody, BLAST-1 antibody, BLAST1 antibody, Bcm-1 antibody, MEM-102 antibody, SLAMF2 antibody, Sgp-60 antibody, hCD48 antibody, mCD48 antibody, CD48 antigen antibody, Cd48 molecule antibody, CD48 molecule antibody, Cd48 antibody, CD48 antibody

背景

CD48 Molecule,CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily, which includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others. These molecules bind to the same or another members of their family, thus mediate homotypic or heterotypic adhesion. CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2. 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation. Signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases.,BCM1, BLAST, BLAST1, SLAMF2

基因ID

962

UniProt

P09326