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CD14 抗体

Name: CD14 抗体
Brand: antibodies-online
SKU: ABIN93971
Availability: OutOfStock

CD14 适用: 人, Non-Human Primate FACS, IP 宿主: 小鼠 Monoclonal MEM-15 unconjugated

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD14 (MEM-15) purified antibody (concentration in sample 0,6 μg/mL, GAM APC).

FACS

Surface staining of human peripheral blood cells with anti-human CD14 (MEM-15) PE.

FACS

Separation of human monocytes (red-filled) from CD14 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD14 (MEM-15) purified antibody (concentration in sample 0,6 μg/mL, GAM APC).

FACS

3 images

(5 references)

产品编号 ABIN93971

发货至: 中国

Datasheet as PDF

抗原 See all CD14 抗体

CD14
适用
246

47

30

29

28

28

25

24

22

18

15

14

10

9

8

8

8

5

2

2

2

1

1

1
人, Non-Human Primate
宿主
204

63

16

3

1
小鼠
Compatible Secondaries
克隆类型
223

64
单克隆
标记
120

29

22

17

13

6

5

4

4

4

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD14 antibody is un-conjugated
应用范围
202

98

61

52

46

44

22

21

20

14

13

13

7

4

4

3

3

3

3

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), Immunoprecipitation (IP)

特异性

The antibody MEM-15 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked extracellular membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes, also expressed by most tissue macrophages. The antibody MEM-15 also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients.

交叉反应（详细）

Human, Non-Human Primates

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.

克隆位点

MEM-15

亚型

IgG1

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anti-CD14 (CD14) (AA 25-148) antibody
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anti-CD14 (CD14) antibody
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应用备注

Immunoprecipitation: Excellent for immunoprecipitation of CD14.
Flow cytometry: Recommended dilution: 4 μg/mL.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Cejkova, Nemeckova, Broz, Cerna: "TLR2 and TLR4 expression on CD14(++) and CD14(+) monocyte subtypes in adult-onset autoimmune diabetes." in: Biomedical papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia, (2015) (PubMed).

Majer, Vlaskova, Krol, Kalina, Kubanek, Stolnaya, Dvorakova, Elleder, Sikora: "Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency." in: Gene, Vol. 498, Issue 2, pp. 183-95, (2012) (PubMed).

Sing, Rost, Tvardovskaia, Roggenkamp, Wiedemann, Kirschning, Aepfelbacher, Heesemann: "Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression." in: The Journal of experimental medicine, Vol. 196, Issue 8, pp. 1017-24, (2002) (PubMed).

Funda, Tucková, Farré, Iwase, Moro, Tlaskalová-Hogenová: "CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited." in: Infection and immunity, Vol. 69, Issue 6, pp. 3772-81, (2001) (PubMed).

Bazil, Horejsí, Baudys, Kristofová, Strominger, Kostka, Hilgert: "Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen." in: European journal of immunology, Vol. 16, Issue 12, pp. 1583-9, (1987) (PubMed).
抗原

CD14

别名

CD14 (CD14 产品)

别名

CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

背景

CD14 Molecule,CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where it serves as a multifunctional lipopolysaccharide receptor. It is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.,LPS-receptor, LPSR, MCLR

基因ID

929

UniProt

P08571

途径

TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades