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BrdU 抗体

BrdU FACS, IHC (p), ICC 宿主: 小鼠 Monoclonal MoBu-1 unconjugated
产品编号 ABIN93883
发货至: 中国
  • 抗原 See all BrdU 抗体
    BrdU (Bromodeoxyuridine (BrdU))
    适用
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    宿主
    • 36
    • 22
    • 6
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    • 1
    小鼠
    克隆类型
    • 44
    • 23
    单克隆
    标记
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    This BrdU antibody is un-conjugated
    应用范围
    • 26
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    Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
    特异性
    The antibody MoBu-1 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It reacts also specifically with 5-bromouridine (BrU), and it does not cross-react with EdU.
    纯化方法
    Purified by protein-A affinity chromatography.
    纯度
    > 95 % (by SDS-PAGE)
    免疫原
    5-bromodeoxyuridine conjugated with hemocyanine.
    克隆位点
    MoBu-1
    亚型
    IgG1
    Top Product
    Discover our top product BrdU Primary Antibody
  • 应用备注
    Flow cytometry: Recommended dilution: 1-2 μg/mL. Intracellular staining.
    Immunocytochemistry: Recommended dilution: 2 μg/mL.
    Immunohistochemistry (paraffin sections): Excellent.
    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not freeze.
    储存条件
    4 °C
    储存方法
    Store at 2-8°C. Do not freeze.
  • Sati, Bonev, Szabo, Jost, Bensadoun, Serra, Loubiere, Papadopoulos, Rivera-Mulia, Fritsch, Bouret, Castillo, Gelpi, Orozco, Vaillant, Pellestor, Bantignies, Marti-Renom, Gilbert, Lemaitre, Cavalli: "4D Genome Rewiring during Oncogene-Induced and Replicative Senescence." in: Molecular cell, Vol. 78, Issue 3, pp. 522-538.e9, (2020) (PubMed).

    Schoonen, Kok, Wierenga, Bakker, Foijer, Spierings, van Vugt: "Premature mitotic entry induced by ATR inhibition potentiates olaparib inhibition-mediated genomic instability, inflammatory signaling, and cytotoxicity in BRCA2-deficient cancer cells." in: Molecular oncology, Vol. 13, Issue 11, pp. 2422-2440, (2020) (PubMed).

    Babic, Ondrejcakova, Bakos, Racekova, Jezova: "Cell proliferation in the hippocampus and in the heart is modified by exposure to repeated stress and treatment with memantine." in: Journal of psychiatric research, Vol. 46, Issue 4, pp. 526-32, (2012) (PubMed).

    Stanek, Kiss, Raska: "Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription." in: European journal of cell biology, Vol. 79, Issue 3, pp. 202-7, (2000) (PubMed).

    Buckiová, Kubínová, Soukup, Jelínek, Brown: "Hyperthermia in the chick embryo: HSP and possible mechanisms of developmental defects." in: The International journal of developmental biology, Vol. 42, Issue 5, pp. 737-40, (1998) (PubMed).

  • 抗原
    BrdU (Bromodeoxyuridine (BrdU))
    别名
    5-bromodeoxyuridine (BrdU) (BrdU 产品)
    物质类
    Chemical
    背景
    Bromodexyuridine (BrdU) is a thymidine analog, which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.,5-bromodeoxyuridine
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