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Biglycan 抗体 (AA 101-200)

Name: Biglycan 抗体 (AA 101-200)
SKU: ABIN872817
Availability: OutOfStock

BGN 适用: 人, 大鼠, 小鼠, 兔 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-Biglycan Polyclonal Antibody, Unconjugated (ABIN872817) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC (p)

Human endothelial cells labeled with Rabbit Anti-Biglycan Polyclonal Antibody, Unconjugated was used to stain the cell nuclei

IF (cc)

Paraformaldehyde-fixed, paraffin embedded mouse brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Biglycan Polyclonal Antibody (bs-7552R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IHC (p)

3 images

(2 references)

产品编号 ABIN872817

发货至: 中国

Datasheet as PDF

抗原 See all Biglycan (BGN) 抗体

Biglycan (BGN)
抗原表位
15

7

5

5

4

3

3

3

2

2

1

1

1

1

1

1
AA 101-200
适用
49

36

31

17

2

2

2

2

2

1

1

1

1
人, 大鼠, 小鼠, 兔
宿主
64

5

1
兔
Compatible Secondaries
克隆类型
57

13
多克隆
标记
29

14

7

2

2

2

2

2

2

2

1

1

1

1

1

1
This Biglycan antibody is un-conjugated
应用范围
54

19

18

15

12

12

8

7

4

3

3

3

2

2
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, 兔, 大鼠

预测反应

Dog,Cow,Sheep,Pig,Horse

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human Biglycan

亚型

IgG

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anti-Biglycan (BGN) (AA 1-368) antibody
BGN 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 4E1-1G7 unconjugated

anti-Biglycan (BGN) (AA 230-366) antibody
BGN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Biglycan (BGN) (AA 53-166) antibody
BGN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Biglycan (BGN) (C-Term) antibody
Verified BGN 适用: 人, 小鼠 ELISA, IF 宿主: 山羊 Polyclonal unconjugated

anti-Biglycan (BGN) (AA 38-300) antibody
BGN 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Biglycan (BGN) antibody
BGN 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Biglycan (BGN) (AA 148-176) antibody
BGN 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB19735 unconjugated

anti-Biglycan (BGN) (AA 1-368) antibody
BGN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Biglycan (BGN) (C-Term) antibody
BGN 适用: 人, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Biglycan (BGN) (AA 20-230) antibody
BGN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Han, Wang, Jiang, Ma, Tian, Zang, Ma: "An Animal Model of Modic Changes by Embedding Autogenous Nucleus Pulposus inside Subchondral Bone of Lumbar Vertebrae." in: Scientific reports, Vol. 6, pp. 35102, (2016) (PubMed).

Huang, Zou, Shi, Zhang, Pen, Zhang, Gao, Wang: "The effect of electroacupuncture on the extracellular matrix synthesis and degradation in a rabbit model of disc degeneration." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 731395, (2014) (PubMed).
抗原

Biglycan (BGN)

别名

Biglycan (BGN 产品)

别名

DSPG1 antibody, PG-S1 antibody, PGI antibody, SLRR1A antibody, BG antibody, BSPG1 antibody, bgn antibody, zgc:91989 antibody, dspg1 antibody, pg-s1 antibody, slrr1a antibody, xbgn antibody, BGN antibody, zgc:77123 antibody, DKFZp468I2427 antibody, biglycan antibody, biglycan L homeolog antibody, biglycan a antibody, biglycan S homeolog antibody, biglycan b antibody, BGN antibody, Bgn antibody, bgn.L antibody, bgna antibody, bgn.S antibody, bgn antibody, bgnb antibody

背景

Synonyms: PGI, DSPG1, PG-S1, SLRR1A, Biglycan, Bone/cartilage proteoglycan I, BGN
Background: The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The encoded protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein plays a role in assembly of collagen fibrils and muscle regeneration. It interacts with several proteins involved in muscular dystrophy, including alpha-dystroglycan, alpha- and gamma-sarcoglycan and collagen VI, and it is critical for the assembly of the dystrophin-associated protein complex.

基因ID

633

UniProt

P21810

途径

Glycosaminoglycan Metabolic Process