CD200 抗体 (AA 41-140)
(1 reference)
(1 validation)-
- 抗原 See all CD200 抗体
- CD200
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抗原表位
- AA 41-140
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CD200 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 交叉反应
- 人
- 预测反应
- Mouse,Rat
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human CD200
- 亚型
- IgG
- Top Product
- Discover our top product CD200 Primary Antibody
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anti-CD200 (CD200) (Internal Region) antibody
CD200 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD200 (CD200) (AA 31-232) antibodyCD200 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD200 (CD200) antibody (PE)CD200 适用: 人 FACS 宿主: 小鼠 Monoclonal OX-104 PE
anti-CD200 (CD200) antibody (FITC)CD200 适用: 人 FACS 宿主: 小鼠 Monoclonal OX-104 FITC
anti-CD200 (CD200) antibodyCD200 适用: 人 FACS, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal OX-104 unconjugated
anti-CD200 (CD200) (AA 56-257) antibodyCD200 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6E8B11 unconjugated
anti-CD200 (CD200) (AA 31-232) antibodyCD200 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM156 unconjugated
anti-CD200 (CD200) antibody (APC)CD200 适用: 人 FACS 宿主: 小鼠 Monoclonal OX-104 APC
anti-CD200 (CD200) antibodyCD200 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-CD200 (CD200) (N-Term) antibodyCD200 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - 限制
- 仅限研究用
-
- by
- Department of Pathology and Molecular Medicine, McMaster University
- No.
- #101517
- 日期
- 2017.07.11
- 抗原
- CD200
- Lot Number
- AG06145442
- Method validated
- Immunohistochemistry
- Positive Control
- Human 7 week normal karyotype elective termination placenta
- Negative Control
- Placental villus from 7w gestation karyotype-normal pregnancy, stained with KLH antibody ABIN401183
- Notes
Passed. ABIN761396 specifically labels the CD200 in human placental tissue.
- Primary Antibody
- ABIN761396
- Secondary Antibody
- Bond Polymer Refine Detection kit (Leica, DS9800, lot 49232)
- Full Protocol
- Fix human placental tissue in 10% buffered formalin for 24h at RT.
- Process and embed tissue in paraffin.
- Cut paraffin blocks with a Leica CM2255 Microtome into 4µm sections.
- Affix sections to positively charged slides and air dry ON at RT.
- Deway and hydrate the slides on an automated Leica BOND Rx stainer.
- Antigen retrieval on the Leica BOND Rx automated stainer using epitope retrieval buffer 2 (Leica, AR9640, lot ER20172).
- Stain slides with primary
- Rabbit anti-CD200 antibody (AA 45-95) antibody (antibodies-online, ABIN761396, lot 980502W),
- rabbit anti-CD200 Receptor 1-Like (CD200R1L) (AA 150-200) antibody(antibodies-online, ABIN1715098, lot 9A13M60), or
- rabbit anti-KLH antibody (antibodies-online, ABIN401183, lot 304770)
- diluted 1:200 in Power Vision IHC Super Blocker (Leica, PV6122). The staining protocol incorporates a modified Leica standard protocol IHC-F (which omits the post-primary step) and uses the standard times outlined in the machine protocol.
- Stain sections with Bond Polymer Refine Detection kit (Leica, DS9800, lot 49232) containing peroxidase block, post primary antibody, polymer as well as DAB chromogen and hematoxylin counterstain for times outlined in the standard protocol IHC-F.
- Remove slides from the Leica Bond Rx and then dehydrate in ethanol and clear in xylene.
- Mount slides in permount mounting medium (Fisher Scientific, SP15-500, lot 162767).
- After the slide-coverslip edges are dry, scan slides using Imagescope and photograph at 400x.
- Experimental Notes
Several dilutions from 1:200 to 1:400 of ABIN761396 were tested and 1:200 was found to be optimal.
The staining pattern of ABIN761396 matches our results with the rabbit anti-CD200 polyclonal antibody RB846 and observations made by others. It is also consistent with staining with the CD200R1L antibody ABIN1715098).
生效 #101517 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Staining with ABIN761396 at dilutions 1:200, 1:250, 1:300, and 1:400 (A, B, C, and D) using a 7 week elective termination of a pregnancy that tested negative for trisomies (Clark et al. (2017) Am. J. Reprod. Immunol). Optimal staining was obtained at 1:200 dilution. Anti-KLH control (E) at 1:200 was negative.]()
Staining with ABIN761396 at dilutions 1:200, 1:250, 1:300, and 1:400 (A, B, C, and D) using a 7 week elective termination of a pregnancy that tested negative for trisomies (Clark et al. (2017) Am. J. Reprod. Immunol). Optimal staining was obtained at 1:200 dilution. Anti-KLH control (E) at 1:200 was negative.
Full Methods -
- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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-
: "The CD200 tolerance-signaling molecule and its receptor, CD200R1, are expressed in human placental villus trophoblast and in peri-implant decidua by 5 weeks' gestation." in: Journal of reproductive immunology, Vol. 112, pp. 20-3, (2015) (PubMed).
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: "The CD200 tolerance-signaling molecule and its receptor, CD200R1, are expressed in human placental villus trophoblast and in peri-implant decidua by 5 weeks' gestation." in: Journal of reproductive immunology, Vol. 112, pp. 20-3, (2015) (PubMed).
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- 抗原
- CD200
- 别名
- Cd200 (CD200 产品)
- 别名
- MOX1 antibody, MOX2 antibody, MRC antibody, OX-2 antibody, CD200 antibody, Cspmo2 antibody, MRCOX2 antibody, Mox2 antibody, DKFZp459C0716 antibody, OX2 antibody, CD200 molecule antibody, Cd200 molecule antibody, CD200 antigen antibody, CD200 antibody, Cd200 antibody
- 背景
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Synonyms: MRC, MOX1, MOX2, OX-2, OX-2 membrane glycoprotein, CD200, My033
Background: Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
- 基因ID
- 4345
- UniProt
- P41217
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