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Phospholipase C gamma 1 抗体 (pTyr783)

Name: Phospholipase C gamma 1 抗体 (pTyr783)
SKU: ABIN744908
Availability: OutOfStock

PLCG1 适用: 小鼠, 大鼠 WB, ELISA, IF (p), IHC (p), FACS, IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded rat lung, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-PLC gamma 1 (Tyr783) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-PLC gamma 1 (Tyr783) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining

IHC (p)

2 images

(1 reference)

产品编号 ABIN744908

发货至: 中国

Datasheet as PDF

抗原 See all Phospholipase C gamma 1 (PLCG1) 抗体

Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))
抗原表位
39

28

14

10

7

7

6

5

5

4

4

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pTyr783
适用
151

136

126

23

9

6

5

3

3

1

1

1
小鼠, 大鼠
宿主
159

23
兔
Compatible Secondaries
克隆类型
144

38
多克隆
标记
85

11

10

10

10

10

9

9

5

4

3

3

3

3

3

2

1

1
This Phospholipase C gamma 1 antibody is un-conjugated
应用范围
120

52

37

31

27

26

18

17

15

13

6

4

3

1
Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

特异性

This phosphorylation site is homologous across the listed species.

交叉反应

小鼠, 大鼠

预测反应

Human,Dog,Pig,Horse,Chicken

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic phosphopeptide derived from human PLC gamma 1 around the phosphorylation site of Tyr783

亚型

IgG

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Discover our top product PLCG1 Primary Antibody
anti-phospholipase C, gamma 1 (PLCG1) (AA 1192-1291) antibody
PLCG1 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2A2 unconjugated

anti-phospholipase C, gamma 1 (PLCG1) (AA 1091-1290) antibody
PLCG1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-phospholipase C, gamma 1 (PLCG1) (pTyr771) antibody
PLCG1 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-phospholipase C, gamma 1 (PLCG1) (AA 1192-1291) antibody
PLCG1 适用: 人, 小鼠 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 3H1C10 unconjugated

anti-phospholipase C, gamma 1 (PLCG1) (AA 1192-1291) antibody
PLCG1 适用: 人, 大鼠, 猴 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 3C2D11 unconjugated

anti-phospholipase C, gamma 1 (PLCG1) (AA 39-181) antibody
PLCG1 适用: 人, 大鼠 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 2F3D11 unconjugated

anti-phospholipase C, gamma 1 (PLCG1) (pTyr783) antibody
PLCG1 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-phospholipase C, gamma 1 (PLCG1) (AA 39-181) antibody
PLCG1 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 2F3E10 unconjugated

anti-phospholipase C, gamma 1 (PLCG1) (Tyr771) antibody
PLCG1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-phospholipase C, gamma 1 (PLCG1) antibody
PLCG1 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Diaz-Flores, Goldschmidt, Depeille, Ng, Akutagawa, Krisman, Crone, Burgess, Williams, Houseman, Shokat, Sampath, Bollag, Roose, Braun, Shannon: "PLC-? and PI3K link cytokines to ERK activation in hematopoietic cells with normal and oncogenic Kras." in: Science signaling, Vol. 6, Issue 304, pp. ra105, (2013) (PubMed).
抗原

Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

别名

PLC gamma 1 (PLCG1 产品)

别名

NCKAP3 antibody, PLC-II antibody, PLC1 antibody, PLC148 antibody, PLCgamma1 antibody, plc1 antibody, nckap3 antibody, plc-ii antibody, plc148 antibody, plcgamma1 antibody, PPLCA antibody, AI894140 antibody, Cded antibody, Plc-1 antibody, Plc-gamma1 antibody, Plcg-1 antibody, fj46f04 antibody, wu:fj46f04 antibody, wu:fj57a05 antibody, phospholipase C gamma 1 antibody, phospholipase C gamma 1 L homeolog antibody, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 antibody, phospholipase C, gamma 1 antibody, PLCG1 antibody, plcg1.L antibody, LOC100633163 antibody, Plcg1 antibody, plcg1 antibody

背景

Synonyms: PLC1, NCKAP3, PLC-II, PLC148, PLCgamma1, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, PLC-148, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-II, Phospholipase C-gamma-1, PLC-gamma-1, PLCG1
Background: Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.

基因ID

5335

UniProt

P19174

途径

RTK signaling, WNT signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling