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GDNF 抗体 (AA 121-211)

Name: GDNF 抗体 (AA 121-211)
SKU: ABIN736536
Availability: OutOfStock
Rating: 5 (1 reviews)

GDNF 适用: 人, 小鼠, 大鼠, Cow, 兔 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Immunofluorescence validation image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 121-211) antibody (ABIN736536)

Lane 1:U251 lysates and Lane 2: Mouse brain lysates probed with Rabbit Anti-GDNF Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded rat brain labeled with Anti-GDNF Polyclonal Antibody, Unconjugated (ABIN736536) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

IHC

Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti-GDNF Polyclonal Antibody, unconjugated (ABIN736536) at 1: 200 followed by incubation with conjugated secondary antibody and DAB staining

IHC

Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti GDNF Polyclonal Antibody, Unconjugated (ABIN736536) at 1:200 in 4 °C.

SDS

Formalin-fixed and paraffin embedded rat brain labeled with Anti-GDNF Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

IHC (p)

6 images

(1 reference)

(1 validation)

产品编号 ABIN736536

发货至: 中国

Datasheet as PDF

抗原 See all GDNF 抗体

GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
抗原表位
15

9

8

6

4

3

3

3

1

1

1

1

1

1
AA 121-211
适用
78

42

31

15

15

1
人, 小鼠, 大鼠, Cow, 兔
宿主
73

6

4

3
兔
Compatible Secondaries
克隆类型
72

13

1
多克隆
标记
39

10

5

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
This GDNF antibody is un-conjugated
应用范围
76

31

25

18

17

12

12

12

6

6

4

4

4

3

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

Cow, 人, 小鼠, 兔, 大鼠

预测反应

Dog,Cow,Pig,Horse,Chicken

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human GDNF

亚型

IgG
anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) antibody
GDNF 适用: 人 WB, IHC, ELISA, IP, FACS 宿主: 兔 Monoclonal 11D2 unconjugated Recombinant Antibody

anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 142-158), (Middle Region) antibody
GDNF 适用: 人, 大鼠 WB, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 8-36) antibody
GDNF 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) antibody (HRP)
GDNF 适用: 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal HRP

anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) antibody (Atto 594)
GDNF 适用: 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 594

anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) antibody (Atto 488)
GDNF 适用: 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 488

anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) antibody (Atto 390)
GDNF 适用: 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 390

anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 109-211) antibody
GDNF 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) antibody
GDNF 适用: 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) antibody (FITC)
GDNF 适用: 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal FITC

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
生效 #102003 (Immunofluorescence)

by

Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin

No.

#102003

日期

2018.02.12

抗原

GDNF

Lot Number

9F01M8

Method validated

Immunofluorescence

Positive Control

mouse hippocampus and putamen

Negative Control

no primary control

Notes

ABIN736536 works in IF albeit with some background staining that we have been unable to eliminate although using different dilutions to increase the signal-to-noise ratio.

Validation Images

IF staining with ABIN736536 of mouse dorsal root ganglion (DRG, A), the dorsal horn of the spinal cord (B), and the hippocampus CA1 (C) and Putamen (D).
Full Methods
Primary Antibody

ABIN736536

Secondary Antibody

anti-rabbit AF488 conjugated antibody (Life Technologies)

Full Protocol

Perfuse mouse with 4% paraformaldehyde in 0.1M phosphate buffer (PB) pH7.4.
Post-fix spinal cord and brain blocks in the same fixative for additional 2h at RT.
Wash spinal cord and brain blocks several times with PBS.
Cut blocks with a vibratome (Leica, VT1000 S) into 70µm thick transverse sections.
Cut dorsal root ganglions (DRGs) with a cryostat into 17µm thick sections after cryoprotection and glass mounting.
Block free floating vibratome and glass mounted cryostat sections with blocking solution (0.01M PBS5% Normal Goat Serum (NGS; Sigma, G9023, lot SLBV1396), 0.1% Triton X-100 (BioRad, 161-0407, lot 00583) for 1h at RT.
Incubate sections with primary rabbit anti-GDNF antibody (antibodies-online, ABIN736536, lot 9F01M8) diluted 1:100 in blocking solution ON at RT.
Wash sections 4x for 5min with 0.01M PBS.
Incubate sections with secondary goat anti-rabbit AF488 conjugated antibody (Life Technologies) diluted 1:500 in PBS for 1h at RT.
Wash sections 4x for 5min with 0.01M PBS.
Mount sections in Fluoroshield (Sigma, F6182, lot MKCB0153V).

Experimental Notes

Different dilutions were also tested (1:200, 1:500, 1:2000) with or without Triton-X but the 1:100 dilution and the use of Triton-X in the blocking solution gave the best results.
Staining is mainly present in the cytoplasm of small-size neurons in the DRG, consistent with what has been previously described. ABIN736536 stains fibers in superficial laminae of the dorsal horn of the spinal cord. In the hippocampus staining is present in the CA1 both in the pyramidal neurons (cell body and dendrites) and in individual interneurons. In the putamen ABIN736536 stains neuron cell bodies and some dendrites.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Zhang, Cai, Song, Dong, Hou, Lv: "Normalization of ventral tegmental area structure following acupuncture in a rat model of heroin relapse." in: Neural regeneration research, Vol. 9, Issue 3, pp. 301-7, (2014) (PubMed).
抗原

GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

别名

GDNF (GDNF 产品)

别名

gdnf antibody, GDNF antibody, AI385739 antibody, gdnfa antibody, hfb1-gdnf antibody, ATF1 antibody, ATF2 antibody, HFB1-GDNF antibody, HSCR3 antibody, glial cell derived neurotrophic factor antibody, glial cell derived neurotrophic factor a antibody, glial cell line derived neurotrophic factor antibody, glial cell derived neurotrophic factor L homeolog antibody, Gdnf antibody, gdnfa antibody, GDNF antibody, gdnf.L antibody

背景

Synonyms: ATF1, ATF2, HSCR3, HFB1-GDNF, Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF
Background: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

基因ID

2668

UniProt

P39905

途径

RTK signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration