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PD-1 抗体 (AA 201-288)

PDCD1 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN735608
发货至: 中国
  • 抗原 See all PD-1 (PDCD1) 抗体
    PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
    抗原表位
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    AA 201-288
    适用
    • 131
    • 73
    • 24
    • 5
    • 3
    • 1
    人, 小鼠, 大鼠
    宿主
    • 75
    • 60
    • 24
    • 11
    • 9
    • 3
    • 2
    克隆类型
    • 126
    • 55
    • 2
    多克隆
    标记
    • 93
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    • 5
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    This PD-1 antibody is un-conjugated
    应用范围
    • 119
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    Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
    交叉反应
    人, 小鼠, 大鼠
    预测反应
    Cow,Horse,Rabbit
    纯化方法
    Purified by Protein A.
    免疫原
    KLH conjugated synthetic peptide derived from human PD-1
    亚型
    IgG
  • 应用备注
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    限制
    仅限研究用
  • 生效 #104535 (Immunohistochemistry)
    '独立验证'标志
    by
    TRON
    No.
    #104535
    日期
    2023.11.29
    抗原
    PD-1
    Lot Number
    BC04411027
    Method validated
    Immunohistochemistry
    Positive Control

    FFPE human tonsil

    Negative Control
    Notes

    Passed. The PD-1 antibody (AA 201-288) ABIN735608 specifically stains cells within the germinal zone in human tonsil.

    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    ABIN735608
    Secondary Antibody
    HRP-conjugated goat anti-rabbit antibody (medac diagnostics, DPVR-110HRP, Lot 101122)
    Full Protocol
    • Dewax and rehydrate tissue sections on glass slides in a descending xylene and graded alcohol series:
      • xylene 2x for 5 min.
      • 100 % (v/v) ethanol 2x for 5 min.
      • 96 % (v/v) ethanol 2x for 5 min.
      • 70 % (v/v) ethanol 2x for 5 min.
    • Wash slides 2x 5 min with ddH2O.
    • Cook slides for 30 min at 95 °C in citrate buffer pH 6.
    • Wash slides 2x 3 min with ddH2O.
    • Incubate slides in 0.3 % (v/v) H2O2 in PBS for 15 min to quench endogenous peroxidase.
    • Wash slides 3x 3 min in 1X PBS.
    • Block slides in 10 % goat serum in PBS for 30 min at RT.
    • Incubate slides with primary rabbit anti-PD-1 antibody (antibodies-online, ABIN735608, lot BC04411027) diluted 1:250 and 1:500 in 10 % goat serum in PBS ON at 4 °C in a humidified chamber.
    • Wash slides 3x 3 min in 1X PBS.
    • Incubate slides with secondary HRP-conjugated goat anti-rabbit antibody (medac diagnostics, DPVR-110HRP, Lot 101122) ready-to-use in 10 % goat serum in PBS for 30 min at RT in a humidified chamber.
    • Wash slides 3x 3 min in 1X PBS.
    • HRP development.
    • Counterstain slides with hematoxylin solution (Carl Roth, T865.2).
    • Wash slides for 15 min under running tap water.
    • Dehydrate sections in an ascending alcohol-xylene series:
      • 70% ethanol 2x for 10 sec.
      • 96% ethanol 2x for 10 sec.
      • 100% ethanol 2x for 10 sec.
      • xylene 2x for 10 sec.
    • Mount sections with Entellan new mounting medium (Sigma-Aldrich, 1008690500).
    • Dry sections.
    • Image acquisition on an Aperio CS2 Digital Pathology Slide Scanner (Leica), Magnification 40x.
    Experimental Notes
    • Staining with ABIN735608 the signal at 1:250 and 1:500 antibody dilution in human tonsil was sharp and localized in the cells within the germinal zone (as expected).

    • ABIN735608 reactivity was also tested in mouse samples. However, in mouse spleen, the signal was almost absent and very weak at Ab dilution 1:250 and 1:500. No signal was detected in mouse pancreas. Even increasing the Ab concentration to 1:150 dilution, in mouse tumor tissue, ABIN735608 detected a very weak, unclear, and sporadic signal. The quality of the signal does not allow the possibility of background staining to be excluded.

  • 状态
    Liquid
    浓度
    1 μg/μL
    缓冲液
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    储存液
    ProClin
    注意事项
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    有效期
    12 months
  • Zhang, Niyazi, Hong, Tuluwengjiang, Zhang, Zhang, Su, Bao: "Effect of EBI3 on radiation-induced immunosuppression of cervical cancer HeLa cells by regulating Treg cells through PD-1/PD-L1 pathway." in: Tumour biology, Vol. 39, Issue 3, pp. 1010428317692240, (2017) (PubMed).

    Szabó, Gáspár, Dajnoki, Papp, Fábos, Szegedi, Zeher: "Expansion of circulating follicular T helper cells associates with disease severity in childhood atopic dermatitis." in: Immunology letters, Vol. 189, pp. 101-108, (2017) (PubMed).

  • 抗原
    PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
    别名
    PD-1/CD279 (PDCD1 产品)
    别名
    CD279 antibody, PD-1 antibody, PD1 antibody, SLEB2 antibody, hPD-1 antibody, hPD-l antibody, Ly101 antibody, Pdc1 antibody, programmed cell death 1 antibody, PDCD1 antibody, Pdcd1 antibody
    背景

    Synonyms: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, Programmed cell death protein 1, Protein PD-1, PDCD1

    Background: Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.

    基因ID
    5133
    UniProt
    Q15116
    途径
    Cancer Immune Checkpoints
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