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TTF1 抗体 (AA 201-300)

TTF1 适用: 人, 大鼠, 小鼠 WB, FACS, IF (cc), ICC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN728713
发货至: 中国
  • 抗原 See all TTF1 抗体
    TTF1 (Transcription Termination Factor, RNA Polymerase I (TTF1))
    抗原表位
    • 15
    • 7
    • 6
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 201-300
    适用
    人, 大鼠, 小鼠
    宿主
    • 47
    • 8
    克隆类型
    • 44
    • 11
    多克隆
    标记
    • 26
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TTF1 antibody is un-conjugated
    应用范围
    • 32
    • 20
    • 15
    • 12
    • 10
    • 7
    • 6
    • 3
    • 3
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunocytochemistry (ICC)
    交叉反应
    人, 小鼠, 大鼠
    纯化方法
    Purified by Protein A.
    免疫原
    KLH conjugated synthetic peptide derived from human TTF-1
    亚型
    IgG
  • 应用备注
    WB 1:300-5000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    ICC 1:100-500
    限制
    仅限研究用
  • 生效 #028752 (Western Blotting)
    '独立验证'标志
    by
    Alamo Laboratories Inc
    No.
    #028752
    日期
    2013.09.08
    抗原
    Lot Number
    120319
    Method validated
    Western Blotting
    Positive Control
    Brain
    Negative Control
    Liver
    Notes
    A strong band was observed at the expected size in the positive control lysate but not in the negative control lysate.
    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    • Antibody: NK2 Homeobox 1 (NKX2-1) antibody
    • Catalog number: ABIN728713
    • Lot number: 120319
    Secondary Antibody
    • Antibody: Goat anti-Rabbit IgG Antibody (HRP)
    • Lot number: YYDW62W
    Full Protocol
    • 1. Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% Beta-mercaptoethanol at 95°C for 5 minutes prior to loading.
    • 2. 24 µg of boiled extracts were loaded and resolved on a 8-16% SDS-polyacrylamide gel.
    • 3. The Spectra Multicolor Broad Range molecular mass marker (26634 Thermo Scientific) was used as a standard.
    • 4. Proteins were transferred onto PVDF membrane by tank transfer and protein transfer was confirmed with Ponceau S staining.
    • 5. The immunoblot membrane was blocked in PBS containing 3% (W/V) non-fat dry milk at room temperature for 1 hour.
    • 6. The membrane was rinsed with PBS containing 0.05% Tween-20 once.
    • 7. The membrane was immersed with the protein side up in the antibody solution in PBS containing 1% (W/V) non-fat dry milk and incubated for 2 hours at room temperature (~26°C).
    • 8. The membrane was rinsed in PBS containing 0.05% Tween-20 thrice for 10 min each.
    • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in PBS containing 1% (W/V) non-fat dry milk and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
    • 10. The membrane was rinsed in PBS containing 0.05% Tween-20 thrice for 10 min each.
    • 11. The membrane was washed in PBS twice for 30 seconds each.
    • 12. Signals were detected with Pierce ECL Western Blotting Substrate (32109, Thermo Scientific). The blot was scanned for 300 seconds.
    • 13. The membrane was rinsed three times with PBS containing 0.05% Tween-20.
    • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
    • 15. The membrane was washed in PBS containing 0.05% Tween-20 times for 10 min each.
    • 16. Repeated Steps 5-12 with the loading control antibody (for Beta-actin) and its matching secondary antibody.
    Experimental Notes
    None
  • 状态
    Liquid
    浓度
    1 μg/μL
    缓冲液
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    储存液
    ProClin
    注意事项
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    有效期
    12 months
  • Vadasz, Jensen, Moncada, Girard, Zhang, Blanchette, Finck: "Second and third trimester amniotic fluid mesenchymal stem cells can repopulate a de-cellularized lung scaffold and express lung markers." in: Journal of pediatric surgery, Vol. 49, Issue 11, pp. 1554-63, (2014) (PubMed).

    Huang, Li, Lin, Shi, Lin, Li, Xu: "Upregulation of thyroid transcription factor-1 and human leukocyte antigen class I in Hashimoto's disease providing a clinical evidence for possible triggering autoimmune reaction." in: European journal of endocrinology / European Federation of Endocrine Societies, Vol. 164, Issue 5, pp. 795-800, (2011) (PubMed).

  • 抗原
    TTF1 (Transcription Termination Factor, RNA Polymerase I (TTF1))
    别名
    TTF1 (TTF1 产品)
    别名
    TTF-1 antibody, TTF-I antibody, AV245725 antibody, RGD1565673 antibody, transcription termination factor 1 antibody, transcription termination factor, RNA polymerase I antibody, TTF1 antibody, Ttf1 antibody
    背景

    Synonyms: BCH, BHC, NK-2, TEBP, TTF1, NKX2A, T/EBP, TITF1, TTF-1, NKX2.1, Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1, Thyroid-specific enhancer-binding protein, NKX2-1

    Background: Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (By similarity).

    基因ID
    7080
    UniProt
    P43699
    途径
    Thyroid Hormone Synthesis, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
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