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AKT 1/2/3 抗体 (AA 401-479)

适用: 人, 小鼠, 大鼠, Cow, Pig WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN725195
发货至: 中国
  • 抗原 See all AKT 1/2/3 products
    AKT 1/2/3
    抗原表位
    • 25
    • 23
    • 23
    • 23
    • 20
    • 20
    • 18
    • 18
    • 16
    • 16
    • 15
    • 7
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 401-479
    适用
    • 164
    • 152
    • 138
    • 45
    • 31
    • 28
    • 24
    • 15
    • 9
    • 6
    • 4
    • 4
    人, 小鼠, 大鼠, Cow, Pig
    宿主
    • 162
    • 2
    克隆类型
    • 151
    • 13
    多克隆
    标记
    • 41
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 2
    • 2
    • 2
    • 2
    • 2
    This AKT 1/2/3 antibody is un-conjugated
    应用范围
    • 147
    • 92
    • 91
    • 90
    • 35
    • 31
    • 29
    • 20
    • 9
    • 7
    • 6
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    交叉反应
    Cow, 人, 小鼠, Pig, 大鼠
    预测反应
    Dog,Cow,Sheep,Chicken
    纯化方法
    Purified by Protein A.
    免疫原
    KLH conjugated synthetic peptide derived from human AKT1
    亚型
    IgG
  • 应用备注
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    限制
    仅限研究用
  • 生效 #029769 (Flow Cytometry)
    '独立验证'标志
    by
    Flow Cytometry & Cell Separation Facility, Purdue University
    No.
    #029769
    日期
    2014.07.22
    抗原
    Lot Number
    131113
    Method validated
    Flow Cytometry
    Positive Control
    MDA-MB-468 cells - lower expression
    Negative Control
    Notes
    A strong specific signal is observed in both of the positive control cells stained with anti-AKT1 plus secondary antibody compared with isotype, secondary only and unstained cells.
    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    • Antigen: V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
    • Catalog number: ABIN725195
    • Lot number: 131113
    • Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA
    Secondary Antibody
    • Antibody: Goat anti-rabbit IgG-FITC
    • Lot number: 115629
    • Dilution: 1:200 in 1X PBS containing 0.5% BSA
    Full Protocol
    • Control cells were cultured in DMEM + 10% FBS. - Control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
    • Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
    • An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37 °C.
    • Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
    • Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA.
    • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - isotype control antibody + secondary antibody - primary antibody + secondary antibody
    • Cells were incubated for 30 min on ice.
    • Labeled cells were washed twice in PBS containing 0.5% BSA.
    • Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
    • Goat anti-rabbit IgG-FITC secondary antibody (Jackson Immunoresearch) was added at a 1:200 dilution. The cells were incubated for 30 min in the dark on ice.
    • Labeled cells were washed twice in PBS containing 0.5% BSA.
    • Cells were analyzed on a FACSAria III (BD Biosciences) using a blue laser (488 nm excitation / 525 nm emission).
    Experimental Notes
    Nothing to note.
  • 状态
    Liquid
    浓度
    1 μg/μL
    缓冲液
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    储存液
    ProClin
    注意事项
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    有效期
    12 months
  • Liu, Liu, Liu, Ma, Zhao: "Surgical incision induces learning impairment in mice partially through inhibition of the brain-derived neurotrophic factor signaling pathway in the hippocampus and amygdala." in: Molecular pain, Vol. 14, pp. 1744806918805902, (2019) (PubMed).

    Zhang, Zhao, Zhang, Hao, Yu, Min, Li, Ma, Chen, Yi, Tang, Meng, Liu, Wang, Shen, Zhang: "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." in: Chemosphere, Vol. 194, pp. 147-157, (2018) (PubMed).

    Zhao, Zhang, Liu, Zhang, Hao, Li, Chen, Shen, Tang, Min, Meng, Wang, Yi, Zhang: "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." in: Scientific reports, Vol. 6, pp. 37884, (2016) (PubMed).

    Zhang, Huang, Guo, Gou, Hinsdale, Lloyd, Liu: "MicroRNA-26b Modulates the NF-κB Pathway in Alveolar Macrophages by Regulating PTEN." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 195, Issue 11, pp. 5404-14, (2015) (PubMed).

    Liang, Zeng, Wang, Shen, Li, Ma, Ci, Yu, Jia, Sun, Liu, Liu, Li, Yu, Chen, Jia: "Histone demethylase RBP2 induced by Helicobactor Pylori CagA participates in the malignant transformation of gastric epithelial cells." in: Oncotarget, Vol. 5, Issue 14, pp. 5798-807, (2015) (PubMed).

    Li, Liu, Wang, Sun, Ding, Sun, Han, Wang: "Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling." in: Bioscience reports, Vol. 34, Issue 5, (2014) (PubMed).

    Xu, Huang, Xie, Lin, Guo, Zhuang, Lin, Zhou, Li, Huang, Wei, Man, Zhang: "The expression of cytoglobin as a prognostic factor in gliomas: a retrospective analysis of 88 patients." in: BMC cancer, Vol. 13, pp. 247, (2013) (PubMed).

    Tang, Liu, Dong, Li, Li, Lin, Zheng, Hou, Li: "The cardioprotective effects of citric Acid and L-malic Acid on myocardial ischemia/reperfusion injury." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2013, pp. 820695, (2013) (PubMed).

  • 抗原
    AKT 1/2/3
    别名
    AKT1/2/3 (AKT 1/2/3 产品)
    背景

    Synonyms: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, Protein kinase B, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, PKB gamma, RAC-PK-gamma, STK-2, PKBG

    Background: AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility, prohibitin (PHB), playing an important role in cell metabolism and proliferation, and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

    基因ID
    207
    UniProt
    P31749
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