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Tissue factor 抗体 (AA 32-100)

F3 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN708086
发货至: 中国
  • 抗原 See all Tissue factor (F3) 抗体
    Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
    抗原表位
    • 15
    • 10
    • 8
    • 7
    • 6
    • 4
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 32-100
    适用
    • 92
    • 42
    • 37
    • 19
    • 8
    • 5
    • 4
    • 4
    • 2
    • 2
    人, 小鼠, 大鼠, Pig
    宿主
    • 86
    • 30
    • 4
    • 2
    克隆类型
    • 80
    • 41
    • 1
    多克隆
    标记
    • 54
    • 21
    • 10
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Tissue factor antibody is un-conjugated
    应用范围
    • 92
    • 37
    • 35
    • 31
    • 18
    • 14
    • 13
    • 12
    • 11
    • 8
    • 7
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    交叉反应
    人, 小鼠, Pig, 大鼠
    预测反应
    Dog,Cow,Horse,Rabbit,Guinea Pig
    纯化方法
    Purified by Protein A.
    免疫原
    KLH conjugated synthetic peptide derived from human CD142
    亚型
    IgG
  • 应用备注
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    限制
    仅限研究用
  • 生效 #029589 (Immunohistochemistry)
    '独立验证'标志
    by
    Reveal Biosciences
    No.
    #029589
    日期
    2014.02.07
    抗原
    Lot Number
    980886W
    Method validated
    Immunohistochemistry
    Positive Control
    Mouse lung tissue
    Negative Control
    Mouse brain tissue
    Notes
    Coagulation Factor III (thromboplastin, Tissue Factor) (F3) staining is observed in positive control tissue and not in negative control tissue.
    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    • Antigen: Coagulation Factor III (thromboplastin, Tissue Factor) (F3)
    • Catalog number: ABIN708086
    • Lot number: 120224
    Secondary Antibody
    • Antibody: Bond Polymer Refine Detection Kit
    • Lot number: 24144
    Full Protocol
    • Immunohistochemistry was performed on a Leica Bond automated immunostainer.
    • Sections were deparaffinized with Novocastra Bond Dewax Solution and rehydrated into Leica Bond Wash Buffer.
    • Sections were heated to 98 °C for 20 minutes in 10 mM citrate buffer pH 9.0 (ER1; Leica) for antigen retrieval.
    • Sections were blocked in 3 % casein plus 0.1 % Triton-X100 for 10 minutes at room temperature.
    • Sections were washed x 3 in Leica Bond Wash Buffer.
    • Sections were incubated with primary antibody diluted 1:200 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) for 15 minutes at room temperature.
    • Sections were washed x 3 in Leica Bond Wash Buffer.
    • Sections were incubated with Leica Bond Polymer for 8 minutes at room temperature.
    • Sections were washed x 4 in Leica Bond Wash Buffer.
    • Sections were washed x 1 in Distilled Water.
    • Sections were incubated with Peroxide Block (Leica) for 10 min to block endogenous peroxidase.
    • Sections were washed x 4 in Leica Bond Wash Buffer.
    • Sections were incubated with DAB chromogenic substrate (Leica) for 10 min at RT.
    • Sections were washed x 3 in Distilled Water.
    • Sections were counterstained with hematoxylin (Leica) for 2 minutes.
    • Sections were washed x 1 in Distilled Water.
    • Sections were washed x 1 in Leica Bond Wash Buffer.
    • Sections were washed x 1 in Distilled Water.
    • Sections were dehydrated, mounted and photographed under a light microscope.
    Experimental Notes
    None notes.
  • 状态
    Liquid
    浓度
    1 μg/μL
    缓冲液
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    储存液
    ProClin
    注意事项
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    有效期
    12 months
  • Fletcher, Das, Gadye, Street, Baudhuin, Wagner, Cole, Flores, Choi, Yosef, Purdom, Dudoit, Risso, Ngai: "Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution." in: Cell stem cell, Vol. 20, Issue 6, pp. 817-830.e8, (2017) (PubMed).

    Kim, Yoo, Gu, Kim: "Histones Induce the Procoagulant Phenotype of Endothelial Cells through Tissue Factor Up-Regulation and Thrombomodulin Down-Regulation." in: PLoS ONE, Vol. 11, Issue 6, pp. e0156763, (2016) (PubMed).

    Yoo, Kim, Gu, Lee, Lee, Hwang, Hwang, Kim, Kim: "Porcine endothelium induces DNA-histone complex formation in human whole blood: a harmful effect of histone on coagulation and endothelial activation." in: Xenotransplantation, Vol. 23, Issue 6, pp. 464-471, (2016) (PubMed).

    Gleeson, Martin, Ali, Kumar, Pillai, Kumar, OSullivan, Whelan, Stocca, Khider, Barry, OBrien, Caplice: "Bone Marrow-Derived Mesenchymal Stem Cells Have Innate Procoagulant Activity and Cause Microvascular Obstruction Following Intracoronary Delivery: Amelioration by Antithrombin Therapy." in: Stem cells (Dayton, Ohio), (2015) (PubMed).

    Tian, Salsbery, Wang, Yuan, Yang, Zhao, Wu, Zhang, Konkle, Thiagarajan, Li, Zhang, Dong: "Brain-derived microparticles induce systemic coagulation in a murine model of traumatic brain injury." in: Blood, (2015) (PubMed).

    Milano, Dongiovanni, Artoni, Gatti, Rosso, Colombo, Bollati, Maggioni, Mannucci, Bertazzi, Fargion, Valenti: "Particulate matter phagocytosis induces tissue factor in differentiating macrophages." in: Journal of applied toxicology : JAT, (2015) (PubMed).

    Liu, Wei, Zhang, Xu, Wang, Zhou: "Procoagulant activity and cellular origin of microparticles in human amniotic fluid." in: Thrombosis research, Vol. 133, Issue 4, pp. 645-51, (2014) (PubMed).

  • 抗原
    Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
    别名
    CD142 (F3 产品)
    别名
    CD142 antibody, TF antibody, TFA antibody, PRO1557 antibody, PRO2086 antibody, TFQTL1 antibody, f3 antibody, AA409063 antibody, Cf-3 antibody, Cf3 antibody, tf antibody, zgc:112151 antibody, coagulation factor III, tissue factor antibody, transferrin antibody, coagulation factor IIIa antibody, coagulation factor III antibody, tissue factor antibody, coagulation factor IIIb antibody, coagulation factor III (thromboplastin, tissue factor) S homeolog antibody, F3 antibody, TF antibody, f3a antibody, tf antibody, f3b antibody, f3.S antibody
    背景

    Synonyms: TF, TFA, CD142, Tissue factor, Coagulation factor III, Thromboplastin, F3

    Background: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

    基因ID
    2152
    UniProt
    P13726
    途径
    Positive Regulation of Endopeptidase Activity, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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