Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
产品特性
XRCC1 (X-ray repair complementing defective repair in Chinese hamster cells 1) corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. XRCC1 is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase (PARP) to participate in the base excision repair (BER) pathway. XRCC1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples.
纯化方法
Affinity Purified
免疫原
This antibody was raised against a recombinant protein corresponding to the N-terminal region of human XRCC1.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
抗原
XRCC1
(X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1))