AATF/Che-1, or Apoptosis-antagonizing Transcription Factoris a key regulator of apoptosis through its interaction with MAP3K12 (DLK), as well as regulation of gene transcription and cell profileration through interaction with RNA polymerase II. AATF/Che-1 may promote cell proliferation by acting as a competitor of histone deacetylase 1 (HDAC1) interaction with the growth inhibiting protein pRb. AATF / Che-1 binding to pRb may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. AATF / Che-1 antibody (mAb) (Clone 1B2D8) was raised in a Rat host. It has been validated for use in Immunofluorescence and Western blot, it has been shown to react with Human and Mouse samples.
纯化方法
Protein A Chromatography
免疫原
This antibody was raised against peptide comprising amino acids 1-39 of mouse AATF (Apoptosis-antagonizing transcription factor) (NP_062790).
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.