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Recombinant MHC Class II HLA-DP/DQ/DR 抗体

HLA-DP/DQ/DR 适用: 人 FACS, IF, WB, IHC, Coat, ELISA, StM 宿主: 兔 Monoclonal HLA-Pan-2967R unconjugated Recombinant Antibody
产品编号 ABIN6941452
发货至: 中国
  • 抗原 See all MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR) products
    MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)
    抗体类型
    Recombinant Antibody
    适用
    • 17
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    宿主
    • 16
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    克隆类型
    • 17
    单克隆
    标记
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    This MHC Class II HLA-DP/DQ/DR antibody is un-conjugated
    应用范围
    • 14
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    Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC), Coating (Coat), ELISA, Staining Methods (StM)
    特异性
    Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36 kDa) and a chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.
    纯化方法
    Purified by Protein A/G
    免疫原
    Non-T, non-B human acute lymphoblastic leukemia REH6 cell line
    克隆位点
    HLA-Pan-2967R
    亚型
    IgG
  • 应用备注

    Positive Control: Ramos or Raji cells. Tonsil or lymph node.

    Known Application: ELISA (For coating use Ab at 2-5 μg/mL, order Ab without BSA), Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL). Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

    限制
    仅限研究用
  • 浓度
    200 μg/mL
    缓冲液
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-80 °C
    储存方法
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    有效期
    24 months
  • 抗原
    MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)
    别名
    HLA-DP, HLA-DQ, HLA-DR (HLA-DP/DQ/DR 产品)
    分子量
    33-35kDa
    基因ID
    3115, 3117, 3122
    UniProt
    P04440, P01908, P01909, P01920, P01903
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