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eIF4EBP1 抗体 (pThr37, pThr46)

Name: eIF4EBP1 抗体 (pThr37, pThr46)
SKU: ABIN682978
Availability: OutOfStock

EIF4EBP1 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated

Mouse ovary and Rat ovary lysates probed with eIF4EBP1 (Thr37+Thr46) Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Formalin-fixed and paraffin embedded mouse stomach tissue labeled with Anti-phospho-eIF4EBP1(Thr37/46) Polyclonal Antibody, Unconjugated (ABIN682978) 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Formalin-fixed and paraffin embedded rat colitis tissue labeled with Anti-phospho-eIF4EBP1 (Thr37/46) Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-phospho-eIF4EBP1(Thr37/46) Polyclonal Antibody, Unconjugated (ABIN682978) followed by conjugation to the secondary antibody and DAB staining

IHC

Paraformaldehyde-fixed, paraffin embedded mouse small intestine tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-eIF4EBP1 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining Image on the left is the same conditions, with the peptide incubated with the antibody prior to staining.

IHC (p)

5 images

产品编号 ABIN682978

发货至: 中国

Datasheet as PDF

抗原 See all eIF4EBP1 (EIF4EBP1) 抗体

eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))
抗原表位
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pThr37, pThr46
适用
236

164

121

21

14

7

6

5

4

3

2

2

1

1

1
人, 小鼠
宿主
255

13

4
兔
Compatible Secondaries
克隆类型
236

38
多克隆
标记
115

25

18

11

10

10

10

9

9

9

7

6

6

6

6

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1

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This eIF4EBP1 antibody is un-conjugated
应用范围
216

90

86

66

65

45

29

23

15

12

10

8

5

3

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠

预测反应

Rat,Dog,Cow,Pig,Horse,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic phosphopeptide derived from human 4EBP1 around the phosphorylation site of Thr37/46

亚型

IgG

Top Product

Discover our top product EIF4EBP1 Primary Antibody
anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) antibody
EIF4EBP1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) (C-Term) antibody
EIF4EBP1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) (pSer65) antibody
EIF4EBP1 适用: 人 WB, IHC, ELISA, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 1E2E7 unconjugated

anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) (pSer65) antibody
EIF4EBP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) (AA 1-118) antibody
EIF4EBP1 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 4F3-H2 unconjugated

anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) (pThr36) antibody
EIF4EBP1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) (AA 2-111) antibody
EIF4EBP1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) (AA 2-118) antibody
EIF4EBP1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) (Thr69) antibody
EIF4EBP1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) (pThr45) antibody
EIF4EBP1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

别名

e4EBP1 + (EIF4EBP1 产品)

别名

4E-BP1 antibody, 4EBP1 antibody, BP-1 antibody, PHAS-I antibody, 4e-bp1 antibody, AA959816 antibody, 153432_at antibody, 43-BP antibody, 4E-BP antibody, 4EBP antibody, 4e-bp antibody, 4eBP antibody, 4ebp antibody, BcDNA.HL08053 antibody, BcDNA:HL08053 antibody, CG8846 antibody, CT8705 antibody, Dm 4E-BP antibody, Dmel\\CG8846 antibody, Phas1 antibody, Thor/EIF4EBP antibody, anon-WO0172774.158 antibody, d4E-BP antibody, d4E-BP1 antibody, d4EBP antibody, d4e-bp antibody, d4eBP antibody, dthor antibody, eIF4E-BP antibody, l(2)06270 antibody, l(2)k07736 antibody, l(2)k13517 antibody, pp20 antibody, thor antibody, EIF4EBP1 antibody, fc04h09 antibody, wu:fc04h09 antibody, zgc:64137 antibody, eukaryotic translation initiation factor 4E binding protein 1 antibody, CG8846 gene product from transcript CG8846-RA antibody, EIF4EBP1 antibody, Eif4ebp1 antibody, Thor antibody, eif4ebp1 antibody

背景

Synonyms: BP-1, 4EBP1, 4E-BP1, PHAS-I, Eukaryotic translation initiation factor 4E-binding protein 1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, EIF4EBP1
Background: Regulates eIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

基因ID

1978

UniProt

Q13541

途径

MAPK Pathway, PI3K-Akt Signaling, RTK signaling, AMPK Signaling, Regulation of Cell Size, BCR Signaling