PRKAA2 抗体 (AA 351-450)
(2 references)-
- 抗原 See all PRKAA2 抗体
- PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))
-
抗原表位
- AA 351-450
-
适用
- 人, 小鼠, 大鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This PRKAA2 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 交叉反应
- 人, 小鼠, 大鼠
- 预测反应
- Dog,Cow,Sheep,Pig,Rabbit
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human AMPK alpha 2
- 亚型
- IgG
- Top Product
- Discover our top product PRKAA2 Primary Antibody
-
anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (AA 453-552) antibody
PRKAA2 适用: 人 WB, ELISA, IF, RNAi, PLA 宿主: 小鼠 Monoclonal 1G8 unconjugated
anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (Center) antibodyPRKAA2 适用: 人, 小鼠 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (AA 453-552) antibodyPRKAA2 适用: 人, 猴 WB, ELISA, IHC, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 8E11H5 unconjugated
anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) antibodyPRKAA2 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (AA 1-552) antibodyPRKAA2 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (AA 350-400), (Catalytic Subunit alpha) antibodyPRKAA2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (AA 265-294) antibodyPRKAA2 适用: 人 WB 宿主: 兔 Polyclonal RB15728 unconjugated
anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (Internal Region) antibodyVerified PRKAA2 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (AA 343-552) antibodyPRKAA2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (pThr172) antibodyPRKAA2 适用: 人 WB, ELISA, IF 宿主: 兔 Monoclonal 4F4 unconjugated Recombinant Antibody
-
- 应用备注
-
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
-
-
: "Metformin ameliorates hepatic steatosis and improves the induction of autophagy in HFD‑induced obese mice." in: Molecular medicine reports, Vol. 16, Issue 1, pp. 680-686, (2017) (PubMed).
: "AMP-activated protein kinase stimulates Warburg-like glycolysis and activation of satellite cells during muscle regeneration." in: The Journal of biological chemistry, Vol. 290, Issue 44, pp. 26445-56, (2015) (PubMed).
-
: "Metformin ameliorates hepatic steatosis and improves the induction of autophagy in HFD‑induced obese mice." in: Molecular medicine reports, Vol. 16, Issue 1, pp. 680-686, (2017) (PubMed).
-
- 抗原
- PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))
- 别名
- AMPK alpha 2 (PRKAA2 产品)
- 别名
- AMPK antibody, AMPK2 antibody, AMPKa2 antibody, PRKAA antibody, Ampk antibody, Ampka2 antibody, 2310008I11Rik antibody, A830082D05 antibody, AMPKalpha2 antibody, AMPKA2 antibody, ampk antibody, ampk2 antibody, prkaa antibody, prkaa1 antibody, PRKAA2 antibody, protein kinase AMP-activated catalytic subunit alpha 2 antibody, protein kinase, AMP-activated, alpha 2 catalytic subunit antibody, protein kinase, AMP-activated, alpha 2 catalytic subunit S homeolog antibody, PRKAA2 antibody, Prkaa2 antibody, prkaa2.S antibody, prkaa2 antibody
- 背景
-
Synonyms: AMPK, AMPK2, PRKAA, AMPKa2, 5'-AMP-activated protein kinase catalytic subunit alpha-2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, PRKAA2
Background: Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE, regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2.
- 基因ID
- 5563
- UniProt
- P54646
- 途径
- AMPK Signaling, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, Warburg Effect
-
