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MAPK14 抗体 (pThr180, pTyr182)

MAPK14 适用: 人, 小鼠, 大鼠, 小鸡, 山羊 WB, ELISA, IHC (p), FACS, IF (cc) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN678668
发货至: 中国
  • 抗原 See all MAPK14 抗体
    MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
    抗原表位
    • 45
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    pThr180, pTyr182
    适用
    • 254
    • 148
    • 130
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    • 15
    • 14
    • 14
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    • 9
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    人, 小鼠, 大鼠, 小鸡, 山羊
    宿主
    • 220
    • 35
    • 2
    • 1
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    克隆类型
    • 201
    • 60
    多克隆
    标记
    • 140
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    This MAPK14 antibody is un-conjugated
    应用范围
    • 205
    • 114
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    • 11
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    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc))
    交叉反应
    小鸡, 山羊, 人, 小鼠, 大鼠
    预测反应
    Dog,Pig,Horse,Rabbit
    纯化方法
    Purified by Protein A.
    免疫原
    KLH conjugated synthetic phosphopeptide derived from human p38 MAPK around the phosphorylation site of(Thr180/Tyr182)
    亚型
    IgG
  • 应用备注
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IF(ICC) 1:50-200
    限制
    仅限研究用
  • 生效 #029662 (Immunohistochemistry)
    '独立验证'标志
    by
    Immunohistochemistry Core, NYU Langone
    No.
    #029662
    日期
    2014.04.09
    抗原
    Lot Number
    140106
    Method validated
    Immunohistochemistry
    Positive Control
    Human placenta tissue
    Negative Control
    Human tonsil tissue (no literature evidence of phospho-p38 MAPK in tonsil)
    Notes
    Signal is detected in positive control tissue and not in negative control tissue.
    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    • Antigen: Mitogen-Activated Protein Kinase 14 (MAPK14) (pThr180), (pTyr182)
    • Catalog number: ABIN678668
    • Lot number: 140106
    Secondary Antibody
    • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
    • Lot number: D07640BA
    Full Protocol
    • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
    • 1. Slides were preheated in convection oven at 60°C for 30 min
    • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
    • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
    • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
    • 5. Slides rinsed with *reaction buffer* x3
    • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
    • 7. Slides rinsed with *reaction buffer*
    • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
    • 9. Slides rinsed with *reaction buffer* x3
    • 10. *iView Biotinylated IgG* applied and incubated for 8 min
    • 11. Slides rinsed with *reaction buffer*
    • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
    • 15. Slides rinsed with *reaction buffer*
    • 16. *iView DAB/H2O2* applied and incubated for 8 min
    • 17. Slides rinsed with *reaction buffer*
    • 18. *iView Copper* applied and incubated for 4 min
    • 19. Slides rinsed with *reaction buffer*
    • 20. Slides washed in Dawn Detergent/tap water
    • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
    • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
    • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
    Experimental Notes
    • Deviations from protocol/procedure supplied by manufacturer:
    • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
    • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
    • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
    • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
    • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
    • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
    • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
    • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
  • 状态
    Liquid
    浓度
    1 μg/μL
    缓冲液
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    储存液
    ProClin
    注意事项
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    有效期
    12 months
  • Iriyama, Hatta, Takei: "Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes." in: Cancer medicine, Vol. 5, Issue 11, pp. 3223-3234, (2016) (PubMed).

    Li, Yu, Liu, Zhu, Hua: "Expression of miR-34c in response to overexpression of Boule and Stra8 in dairy goat male germ line stem cells (mGSCs)." in: Cell biochemistry and function, Vol. 31, Issue 4, pp. 281-8, (2013) (PubMed).

    Wang, Sun, Liu, Yu, Li, Zeng, Chen, Jia: "Upregulation of progranulin by Helicobacter pylori in human gastric epithelial cells via p38MAPK and MEK1/2 signaling pathway: role in epithelial cell proliferation and migration." in: FEMS immunology and medical microbiology, Vol. 63, Issue 1, pp. 82-92, (2012) (PubMed).

  • 抗原
    MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
    别名
    p38 MAPK + (MAPK14 产品)
    别名
    CSBP antibody, CSBP1 antibody, CSBP2 antibody, CSPB1 antibody, EXIP antibody, Mxi2 antibody, PRKM14 antibody, PRKM15 antibody, RK antibody, SAPK2A antibody, p38 antibody, p38ALPHA antibody, CRK1 antibody, Csbp1 antibody, Csbp2 antibody, Exip antibody, Hog antibody, Prkm14 antibody, Prkm15 antibody, Sapk2A antibody, p38Hog antibody, p38alpha antibody, p38b antibody, zp38b antibody, MAPK14 antibody, 186F5S antibody, BG:DS00797.3 antibody, CG7393 antibody, D-p38 antibody, D-p38 MAPK antibody, D-p38b antibody, Dm p38b antibody, Dmel\\CG7393 antibody, Dmp38b antibody, Dp38 antibody, Dp38b antibody, ESTS:186F5S antibody, Mpk34C antibody, anon-sts23 antibody, dp38b antibody, p38 MAPK antibody, p38 beta antibody, p38B antibody, p38Kb antibody, p38beta antibody, Crk1 antibody, p38-alpha antibody, p38MAPK antibody, p38a antibody, csbp antibody, mapk14a antibody, mxi2 antibody, sapk2 antibody, sapk2a antibody, AP22.98 antibody, AP22_98 antibody, ATMPK14 antibody, mitogen-activated protein kinase 14 antibody, SAPK2a antibody, MAP kinase 14A antibody, MAP kinase p38a antibody, MAPK 14A antibody, fk28c03 antibody, hm:zeh1243 antibody, wu:fk28c03 antibody, zp38a antibody, P38C-CRK antibody, mitogen-activated protein kinase 14 antibody, mitogen activated protein kinase 14 antibody, mitogen-activated protein kinase 14b antibody, p38b MAP kinase antibody, mitogen-activated protein kinase 14 S homeolog antibody, mitogen-activated protein kinase 14a antibody, CRK proto-oncogene, adaptor protein antibody, MAPK14 antibody, Mapk14 antibody, mapk14b antibody, p38b antibody, mapk14.S antibody, MPK14 antibody, mapk14a antibody, CRK antibody
    背景

    Synonyms: RK, p38, CSBP, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA, Mitogen-activated protein kinase 14, MAP kinase 14, MAPK 14, Cytokine suppressive anti-inflammatory drug-binding protein, CSAID-binding protein, MAP kinase MXI2, MAX-interacting protein 2, Mitogen-activated protein kinase p38 alpha, MAP kinase p38 alpha, Stress-activated protein kinase 2a, MAPK14

    Background: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

    基因ID
    1432
    UniProt
    Q16539
    途径
    MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins
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