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GAPDH 抗体 (AA 1-335)

Name: GAPDH 抗体 (AA 1-335)
SKU: ABIN678458
Availability: OutOfStock

GAPDH 适用: 人, 小鼠, 大鼠, 兔, Pig, 小鸡, Cow, Various Species WB, IHC (p), FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Western Blotting (WB) image for anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibody (ABIN678458)

Paraformaldehyde-fixed, paraffin embedded Mouse brain Antigen retrieval by boiling in sodium citrate buffer (pH6.

IHC (p)

Mouse muscle lysates probed with GAPDH Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

U251 lysates probed with GAPDH Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

Lane 1: Rat muscle lysates Lane 2: Rat heart lysates probed with GAPDH Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

Lane 1: Hela lysates Lane 2: MCF-7 lysates probed with GAPDH Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

Western blot analysis of SOX2, NANOG, and OCT protein.

7 images

(16 references)

产品编号 ABIN678458

发货至: 中国

Datasheet as PDF

抗原 See all GAPDH 抗体

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
抗原表位
37

20

13

9

7

6

6

5

4

4

3

3

3

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-335
适用
222

141

134

74

64

54

35

28

23

21

20

19

18

15

12

12

11

10

9

8

6

4

4

4

4

3

3

3

3

3

2

2

1

1

1

1

1
人, 小鼠, 大鼠, 兔, Pig, 小鸡, Cow, Various Species
宿主
169

97

17

10

4
兔
Compatible Secondaries
克隆类型
190

105
多克隆
标记
156

36

23

17

5

5

5

5

5

4

4

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1
This GAPDH antibody is un-conjugated
应用范围
244

98

84

70

45

41

40

35

15

15

15

14

8

6

5

4

4

3

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

小鸡, Cow, 人, 小鼠, Pig, 兔, 大鼠, Various Species

纯化方法

Purified by Protein A.

免疫原

GAPDH protein of rabbit

亚型

IgG

Top Product

Discover our top product GAPDH Primary Antibody
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人 WB, IF, IHC (p), ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibody
GAPDH 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody
Verified GAPDH 适用: 人, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibody
GAPDH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C2 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody
Verified GAPDH 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibody
GAPDH 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A10A10 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibody
GAPDH 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB16537 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibody
GAPDH 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibody
GAPDH 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Zhang, Wang, Xie, Chen, Sinkemani, Yu, Wang, Mao, Wu: "Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence." in: Oncotarget, Vol. 9, Issue 2, pp. 2175-2192, (2018) (PubMed).

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Hou, Liu, Chai, Yu, Duan, Hu, Yin, Wang, Zhuang, Fan, Chu, Ma: "Lipopolysaccharide pretreatment inhibits LPS-induced human umbilical cord mesenchymal stem cell apoptosis via upregulating the expression of cellular FLICE-inhibitory protein." in: Molecular medicine reports, Vol. 12, Issue 2, pp. 2521-8, (2015) (PubMed).

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Song, Xiao, Fu, Huang, Wang, Zhang, Yang: "Increased expression of the pluripotency markers sex-determining region Y-box 2 and Nanog homeobox in ovarian endometriosis." in: Reproductive biology and endocrinology : RB&E, Vol. 12, pp. 42, (2014) (PubMed).

Yan, Yang, Li, Duan, Wu, Qian, Li, Wang: "Regulator of calcineurin 1-1L protects cardiomyocytes against hypoxia-induced apoptosis via mitophagy." in: Journal of cardiovascular pharmacology, Vol. 64, Issue 4, pp. 310-7, (2014) (PubMed).

Steenblock, Heckel, Czupalla, Espírito Santo, Niehage, Sztacho, Hoflack: "The Cdc42 guanine nucleotide exchange factor FGD6 coordinates cell polarity and endosomal membrane recycling in osteoclasts." in: The Journal of biological chemistry, Vol. 289, Issue 26, pp. 18347-59, (2014) (PubMed).

Li, McFadden, Morgan, Cui, Hu, Wan, Zeng: "All-trans retinoic acid regulates the expression of the extracellular matrix protein fibulin-1 in the guinea pig sclera and human scleral fibroblasts." in: Molecular vision, Vol. 16, pp. 689-97, (2010) (PubMed).
抗原

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

别名

Gapdh (GAPDH 产品)

别名

G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody

背景

Synonyms: G3PD, GAPD, HEL-S-162eP, Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, CDABP47, OK/SW-cl.12
Background: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

基因ID

2597

UniProt

P04406