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TCF7L1 抗体 (AA 35-84)

TCF7L1 适用: 人, 小鼠, 大鼠, Cow, 兔, 豚鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN6741527
发货至: 中国
  • 抗原 See all TCF7L1 抗体
    TCF7L1 (Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1))
    抗原表位
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 35-84
    适用
    • 34
    • 16
    • 5
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    人, 小鼠, 大鼠, Cow, 兔, 豚鼠
    宿主
    • 32
    • 1
    • 1
    克隆类型
    • 33
    • 1
    多克隆
    标记
    • 18
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This TCF7L1 antibody is un-conjugated
    应用范围
    • 18
    • 9
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    Human TCF7L1
    预测反应
    Percent identity by BLAST analysis: Human, Mouse, Guinea pig (100%) Rabbit (85%).
    纯化方法
    Immunoaffinity purified
    免疫原
    Synthetic peptide located between aa35-84 of human TCF7L1 (Q9HCS4, NP_112573). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Galago, Marmoset, Mouse, Rat, Bovine, Guinea pig (100%), Monkey, Elephant, Opossum (92%), Rabbit (85%).

    Type of Immunogen: Synthetic peptide
    亚型
    IgG
    Top Product
    Discover our top product TCF7L1 Primary Antibody
  • 应用备注
    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Distilled water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS with 2 % sucrose
    注意事项
    Avoid repeat freeze-thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • 抗原
    TCF7L1 (Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1))
    别名
    TCF7L1 (TCF7L1 产品)
    别名
    TCF-3 antibody, TCF3 antibody, Tcf-3 antibody, Tcf3 antibody, bHLHb21 antibody, tcf3c antibody, tcf3d antibody, xtcf3 antibody, XTCF-3 antibody, tcf7l1 antibody, tcf3b antibody, TCF-3-A antibody, tcf-3 antibody, tcf3 antibody, tcf3a antibody, zTcf-3 antibody, transcription factor 7 like 1 antibody, transcription factor 7 like 1 (T cell specific, HMG box) antibody, transcription factor 7 like 1 L homeolog antibody, transcription factor 7 like 1 S homeolog antibody, transcription factor 7 like 1b antibody, transcription factor 7 like 1a antibody, TCF7L1 antibody, Tcf7l1 antibody, tcf7l1.L antibody, tcf7l1.S antibody, tcf7l1b antibody, tcf7l1a antibody
    背景
    Name/Gene ID: TCF7L1

    Synonyms: TCF7L1, TCF-3, Transcription factor 7-like 1, HMG box transcription factor 3
    基因ID
    83439
    NCBI登录号
    NP_112573
    UniProt
    Q9HCS4
    途径
    WNT signaling, Stem Cell Maintenance
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