NFIA 抗体 (Middle Region)
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- 抗原 See all NFIA 抗体
- NFIA (Nuclear Factor I/A (NFIA))
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抗原表位
- AA 180-224, Middle Region
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This NFIA antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
- 原理
- Mouse IgG monoclonal antibody for NFIA detection. Tested with Tested with WB, IHC-P, FCM in Human.
- 序列
- AYFVHAADSS QSESPSQPSD ADIKDQPENG HLGFQDSFVT SGVFS
- 交叉反应 (详细)
- No cross reactivity with other proteins.
- 产品特性
- Mouse IgG monoclonal antibody for NFIA detection. Tested with Tested with WB, IHC-P, FCM in Human.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human NFIA (180-224aa AYFVHAADSSQSESPSQPSDADIKDQPENGHLGFQDSFVTSGVFS), different from the related mouse sequence by one amino acid, and identical to the related rat sequence.
- 克隆位点
- 16H11
- 亚型
- IgG
- Top Product
- Discover our top product NFIA Primary Antibody
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- 应用备注
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Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL Flow Cytometry|1-3 μg/1x106 cells
- 说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- 抗原
- NFIA (Nuclear Factor I/A (NFIA))
- 别名
- NFIA (NFIA 产品)
- 别名
- NFIA antibody, CTF antibody, NF-I/A antibody, NF1-A antibody, NFI-A antibody, NFI-L antibody, si:ch211-88d2.2 antibody, wu:fq27e07 antibody, zgc:158351 antibody, CNFI-A antibody, cNFI-A3 antibody, 1110047K16Rik antibody, 9430022M17Rik antibody, NF1A antibody, nuclear factor I A antibody, nuclear factor I/A antibody, NFIA antibody, nfia antibody, Nfia antibody
- 背景
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Synonyms: Nuclear factor 1 A-type, NF1-A, Nuclear factor 1/A, CCAAT-box-binding transcription factor, CTF, Nuclear factor I/A, NF-I/A, NFI-A, TGGCA-binding protein, NFIA, KIAA1439
Background: Nuclear factor 1 A-type is a protein that in humans is encoded by the NFIA gene. Nuclear factor I (NFI) proteins constitute a family of dimericDNA-binding proteins with similar, and possibly identical, DNA-binding specificity. They function as cellular transcription factors and as replication factors for adenovirus DNA replication. Diversity in this protein family is generated by multiple genes, differential splicing, and heterodimerization.
- 基因ID
- 4774
- UniProt
- Q12857
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