山羊 anti-大鼠 IgM (Chain mu) Antibody (DyLight 649) - Preadsorbed
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- 抗原 See all IgM products
- IgM
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抗原表位
- Chain mu
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适用
- 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- DyLight 649
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应用范围
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- 产品特性
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Synonyms: Goat Anti Rat IgM (mu chain) Antibody DyLight 649™ Conjugated, Goat Anti-Rat IgM mu chain Antibody DyLight 649™ Conjugation
Background: Anti-Rat IgM antibody specifically detects rat IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Rat IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology. This Rat IgM Antibody is conjugated to DyLight™649.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Rat IgM whole molecule
- 亚型
- IgG
- Labeling Ratio
- 3.1
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Goat anti-Rat IgM (Heavy Chain) antibody
ELISA, IHC, FACS, WB Polyclonal IgG unconjugated
Goat anti-Rat IgM (Heavy Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rat IgM (Heavy Chain) antibody (Biotin)ELISA, IHC, FACS, WB Polyclonal IgG Biotin
Rabbit anti-Rat IgM (Heavy Chain) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Goat anti-Rat IgM (Heavy Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated
Rabbit anti-Rat IgM (Heavy Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated
Rabbit anti-Rat IgM (Heavy Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG TRITC
Goat anti-Rat IgM (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Rat IgM (Heavy Chain) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
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- 应用备注
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
FLISA Dilution: >1:20,000
Western Blot Dilution: >1:10,000
IF Microscopy Dilution: >1:5,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
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