山羊 anti-兔 IgG (Heavy & Light Chain) Antibody (DyLight 800) - Preadsorbed
(14 references)-
- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 兔
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- DyLight 800
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应用范围
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- 原理
- Goat anti-Rabbit IgG DyLight™ 800 Conjugated Antibody
- 特异性
- This antibody will react with heavy chains of Rabbit IgG and with light chains of most Rabbit immunoglobulins.
- 产品特性
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Synonyms: Goat anti-Rabbit IgG Antibody DyLight™800 Conjugation, Goat anti-Rabbit IgG DyLight™ 800 Conjugated Antibody
Background: Anti-Rabbit IgG Antibody DyLight™800 generated in goat detects rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to DyLight™800.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
- 免疫原
- Rabbit IgG whole molecule
- 亚型
- IgG
- Labeling Ratio
- 1.7
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Guinea Pig anti-Rabbit IgG (Heavy & Light Chain) antibody - Preadsorbed
ELISA, IHC, WB, CUT&RUN, CUT&Tag Polyclonal IgG unconjugated
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Goat anti-Rabbit IgG antibody (HRP)ELISA, WB IgG HRP
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 425) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 425
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 488) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 488
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 647N) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 647N
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- 应用备注
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
FLISA Dilution: >1:20,000
Western Blot Dilution: >1:10,000
IF Microscopy Dilution: >1:5,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "PHF23 (plant homeodomain finger protein 23) negatively regulates cell autophagy by promoting ubiquitination and degradation of E3 ligase LRSAM1." in: Autophagy, Vol. 10, Issue 12, pp. 2158-70, (2015) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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