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山羊 anti-兔 IgG Antibody (Cy5) - Preadsorbed

WB, FACS, FLISA, FM Polyclonal IgG Cy5
产品编号 ABIN6699092
发货至: 中国
  • 抗原 See all IgG products
    IgG
    适用
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    宿主
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    山羊
    克隆类型
    • 3346
    • 164
    • 1
    多克隆
    标记
    • 556
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    Cy5
    应用范围
    • 1837
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    • 515
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    Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    产品特性

    Synonyms: Goat Anti Rabbit IgG Antibody CY5 Conjugation, Goat Anti-Rabbit IgG CY5 Conjugated Antibody

    Background: Anti-Rabbit IgG Antibody CY5 generated in goat detects rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to CY5.

    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Rabbit IgG whole molecule

    亚型
    IgG
    Labeling Ratio
    17.2
  • 应用备注

    Application Note: This product is designed for flow cytometry, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    Western Blot Dilution: User Optimized

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • Richardson, Winistorfer, Poupon, Luzio, Piper: "Mammalian late vacuole protein sorting orthologues participate in early endosomal fusion and interact with the cytoskeleton." in: Molecular biology of the cell, Vol. 15, Issue 3, pp. 1197-210, (2004) (PubMed).

  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
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