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兔 anti-小鼠 IgG2a (Chain gamma 2a) Antibody (DyLight 649) - Preadsorbed

WB, FLISA, FM Polyclonal IgG DyLight 649
产品编号 ABIN6699064
发货至: 中国
  • 抗原 See all IgG2a products
    IgG2a
    抗原表位
    • 43
    • 14
    • 7
    • 2
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    • 1
    • 1
    Chain gamma 2a
    适用
    • 89
    • 18
    • 1
    小鼠
    宿主
    • 57
    • 22
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    • 5
    克隆类型
    • 71
    • 24
    多克隆
    标记
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    • 1
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    DyLight 649
    应用范围
    • 50
    • 43
    • 34
    • 30
    • 23
    • 18
    • 10
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    • 7
    • 5
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    Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
    产品特性

    Synonyms: Rabbit Anti-Mouse IgG2a (Gamma 2a chain) Antibody DyLight 649™ Conjugated, Rabbit Anti Mouse IgG2a DyLight 649™ Conjugated Antibody

    Background: Anti-Mouse IgG2a DyLight 649 Antibody generated in rabbit detects reactivity to Mouse IgG2a (Gamma 2a chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Mouse IgG2a heavy chain

    亚型
    IgG
    Labeling Ratio
    3.2
  • 应用备注

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

    FLISA Dilution: >1:20,000

    Western Blot Dilution: >1:10,000

    IF Microscopy Dilution: >1:5,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • 抗原
    IgG2a
    Abstract
    IgG2a 产品
    物质类
    Antibody
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